{
  "abstract": "Bacterial transformation of 3 nitropropionic acid (3NPA) is typically viewed through canonical nitronate monooxygenases (NMOs), yet sequence annotation poorly resolves function across this flavoprotein family. Here we show that the environmental YrpB/NMO associated oxidoreductase space is dominated by YrpB related proteins: across 13 metagenomes, 83.1% of 390 conserved domain supported candidates were YrpB-associated. Cultivation along an Eisenia fetida feed gut cast continuum recovered 31 phylogenetically diverse 3NPA responsive bacteria, revealing that this phenotype is distributed across multiple lineages. Using the previously genome-sequenced Serratia sp. EWG9 as a tractable exemplar, we demonstrate sustained 3NPA supported growth, 44% parent compound depletion and a broad early transcriptional reorganization. Its focal oxidoreductase OXR01 occupies overlapping YrpB/NMO sequence space and shows stable predicted 3NPA accommodation without strong transcriptional induction. These findings reposition canonical NMOs as one component of a broader, ecologically distributed oxidoreductase reservoir for bacterial nitrotoxin responsiveness.",
  "abstract_shown": true,
  "affiliations": [
    "University of North Bengal"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "name": "P. Barman"
    },
    {
      "name": "S. Sinha"
    },
    {
      "affs": [
        0
      ],
      "name": "R. Chakraborty"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/avjme1f055n6b.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.13.751228",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.13.751228v1.full",
  "id": "avjme1f055n6b",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.13.751228v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "microbiology"
    ],
    [
      "openalex",
      "2310|Microbial bioremediation and biosurfactants"
    ]
  ],
  "openalex": "W7213442386",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.13.751228v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-13",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/avjme1f055n6b.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:45Z",
      "native_id": "10.64898/2026.09.13.751228",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.13.751228v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:microbiology",
      "subfield": "microbiology"
    }
  ],
  "title": "Nitrotoxin metabolism in bacteria may have emerged from a diverse oxidoreductase reservoir",
  "title_text": "Nitrotoxin metabolism in bacteria may have emerged from a diverse oxidoreductase reservoir",
  "type": "preprint",
  "updated_at": "2026-10-30T12:22:45Z",
  "url": "https://research.draconitic.com/paper/avjme1f055n6b",
  "venue": "bioRxiv",
  "versions": []
}