{
  "abstract": "Luminescence is a powerful method for detecting trace analytes and monitoring biological processes. However, most bioluminescence reagents, including luciferase and its substrates, are sensitive to temperature, limiting their usable shelf lives, and resulting in inconsistent performance. Enhancing the stability of these reagents could reduce signal variability between experiments, simplify workflows, and address cold chain storage issues. In this study, we demonstrate the application of the platform stabilization technology, capillary-assisted vitrification (CAV), as a tool to stabilize different luciferases and their substrates, and the application of the stabilized reagents in both in vitro and in vivo bioluminescent assays. We demonstrate that CAV-stabilized reagents can be stored and shipped ambiently, maintain consistent performance over time, and are suitable for use in cell viability quantification, tumor monitoring, in vivo imaging, microbial detection, and immunoassays. Additionally, different reagents can be co-formulated to make ready-to-use assay kits that can also be shipped and stored ambiently. Our results demonstrate that CAV stabilization is a viable alternative to traditional storage methods, with broad potential to improve bioluminescence workflows.",
  "abstract_shown": true,
  "affiliations": [
    "Ambient Biosciences, Ann Arbor, Michigan, United States of America",
    "Promega Corp., Madison, Wisconsin, United States of America",
    "Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, Tennessee, United States of America",
    "Department of Biomedical Engineering, Vanderbilt University, Nashville, Tennessee, United States of America",
    "Department of Radiation Oncology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 15,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Shari Radford"
    },
    {
      "affs": [
        1
      ],
      "name": "Yolanda Peris-Taverner"
    },
    {
      "affs": [
        2
      ],
      "name": "Michael Dibble"
    },
    {
      "affs": [
        3
      ],
      "name": "Kevin Corn"
    },
    {
      "affs": [
        4
      ],
      "name": "Tian Zhu"
    },
    {
      "name": "Shannon Martello"
    },
    {
      "name": "McKenzie Mayeaux"
    },
    {
      "name": "Amanda Ladd"
    },
    {
      "name": "Sankar Renu"
    },
    {
      "name": "Tejasvi Chunduri"
    },
    {
      "name": "Aniket Jadhav"
    },
    {
      "name": "Melanie Dart"
    },
    {
      "name": "Marjan Rafat"
    },
    {
      "name": "Mary Shank-Retzlaff"
    },
    {
      "name": "Laura L. Bronsart"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/avwa5cjjha82f.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0354533",
  "fulltext_url": null,
  "id": "avwa5cjjha82f",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0354533",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Enzymology/Enzymes/Oxidoreductases/Luciferase"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Proteins/Enzymes/Oxidoreductases/Luciferase"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Proteins/Luminescent proteins/Luciferin"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Proteins/Post-translational modification/Signal peptides"
    ],
    [
      "plos",
      "/Medicine and health sciences/Oncology/Cancers and neoplasms/Breast tumors/Breast cancer"
    ],
    [
      "plos",
      "/Physical sciences/Materials science/Materials/Porous materials"
    ],
    [
      "plos",
      "/Physical sciences/Physics/Electromagnetic radiation/Luminescence/Bioluminescence"
    ],
    [
      "plos",
      "/Research and analysis methods/Immunologic techniques/Immunoassays"
    ],
    [
      "plos",
      "/Research and analysis methods/Specimen preparation and treatment/Tissue dehydration/Lyophilization"
    ]
  ],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-05",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/avwa5cjjha82f.ris",
  "sources": [
    {
      "first_seen": "2026-11-06T12:20:42Z",
      "native_id": "10.1371/journal.pone.0354533",
      "source": "plos",
      "url": "https://doi.org/10.1371/journal.pone.0354533"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "plos:enzymology",
      "subfield": "enzymology-metabolism"
    },
    {
      "field": "chemistry",
      "origin": "plos:materials science",
      "subfield": "materials-polymers"
    },
    {
      "field": "medicine",
      "origin": "plos:cancers and neoplasms",
      "subfield": "oncology"
    }
  ],
  "title": "Development of shelf-stable reagents and assay kits for bioluminescence applications using the capillary-assisted vitrification platform stabilization technology",
  "title_text": "Development of shelf-stable reagents and assay kits for bioluminescence applications using the capillary-assisted vitrification platform stabilization technology",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T12:20:24Z",
  "url": "https://research.draconitic.com/paper/avwa5cjjha82f",
  "venue": "PLOS One",
  "versions": []
}