{
  "abstract": "Calreticulin (CRT) is an endoplasmic reticulum (ER) chaperone with low-affinity calcium-binding sites in its C-terminal domain. This region is altered by somatic mutations in the CRT gene (CALR), which drive a subset of myeloproliferative neoplasms (MPNs). Perturbations in ER calcium storage and signaling are reported for the MPN type I mutant, CRTDel52, and are linked to disease pathogenesis. Using recombinant CRT proteins, we found similar low-affinity calcium-binding characteristics for wild-type CRT and CRTDel52, as determined by isothermal titration calorimetry (ITC). Residues 340–349, conserved in both wild-type CRT and CRTDel52, contribute to binding. Furthermore, ER and cytosolic calcium levels, and store-operated calcium entry (SOCE), were comparable in CRT knockout (CRT-KO) cells reconstituted with wild-type CRT or CRTDel52. Notably, the CRT-KO induces expression of multiple ER proteins known to contain low-affinity calcium-binding sites or regulate ER calcium levels. Overall, these findings indicate that CRTDel52 retains at least some low-affinity calcium-binding capacity and that CRT deficiency induces compensatory cellular changes that maintain ER calcium homeostasis.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, United States",
    "West African Centre for Cell Biology of Infectious Pathogens, Departments of Biochemistry, Cell and Molecular Biology, University of Ghana, Accra, Ghana",
    "Medical Laboratory Sciences, University of Ghana, Accra, Ghana",
    "Department of Pharmacology, University of Michigan Medical School, Ann Arbor, United States"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Ishmael Nii Ayibontey Tagoe",
      "orcid": "0009-0003-4511-6410"
    },
    {
      "affs": [
        0
      ],
      "name": "Amanpreet Kaur",
      "orcid": "0000-0001-6806-8448"
    },
    {
      "affs": [
        1
      ],
      "name": "Osbourne Quaye",
      "orcid": "0000-0002-0621-876X"
    },
    {
      "affs": [
        2
      ],
      "name": "Emmanuel Ayitey Tagoe",
      "orcid": "0000-0001-7179-1872"
    },
    {
      "affs": [
        0
      ],
      "name": "Nicole M Koropatkin",
      "orcid": "0000-0002-2459-3336"
    },
    {
      "affs": [
        3
      ],
      "name": "Leslie S Satin",
      "orcid": "0000-0003-1355-8647"
    },
    {
      "affs": [
        0
      ],
      "name": "Malini Raghavan",
      "orcid": "0000-0002-1345-9318"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/aw5y8a2m8d55g.bib",
  "cited_by": 0,
  "doi": "10.7554/elife.109647",
  "fulltext_url": "https://elifesciences.org/articles/109647",
  "id": "aw5y8a2m8d55g",
  "issns": [
    "2050-084X"
  ],
  "landing_url": "https://elifesciences.org/articles/109647",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "elife",
      "cancer-biology"
    ],
    [
      "elife",
      "cell-biology"
    ],
    [
      "heading",
      "Cancer Biology"
    ],
    [
      "heading",
      "Cell Biology"
    ],
    [
      "heading",
      "Research Article"
    ],
    [
      "openalex",
      "2809|Ion Channels and Receptors"
    ]
  ],
  "openalex": "W7125507970",
  "pdf_url": "https://cdn.elifesciences.org/articles/109647/elife-109647-v1.pdf",
  "pmcid": "PMC13719167",
  "pmid": "42956057",
  "published": "2026-10-29",
  "publisher": "eLife Sciences Publications",
  "ris": "https://research.draconitic.com/paper/aw5y8a2m8d55g.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:25Z",
      "native_id": "109647",
      "source": "elife",
      "url": "https://elifesciences.org/articles/109647"
    },
    {
      "first_seen": "2026-10-31T00:20:39Z",
      "native_id": "MED:42956057",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42956057"
    },
    {
      "first_seen": "2026-11-02T12:23:03Z",
      "native_id": "PMC13719167",
      "source": "pmc",
      "url": "https://doi.org/10.7554/elife.109647"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "elife:cell-biology",
      "subfield": "cell-biology"
    },
    {
      "field": "medicine",
      "origin": "elife:cancer-biology",
      "subfield": "oncology"
    }
  ],
  "title": "Molecular and functional analysis of calcium binding by a cancer-linked calreticulin mutant",
  "title_text": "Molecular and functional analysis of calcium binding by a cancer-linked calreticulin mutant",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:23:03Z",
  "url": "https://research.draconitic.com/paper/aw5y8a2m8d55g",
  "venue": "eLife",
  "versions": []
}