{
  "abstract": "In the process of mounting humoral immune responses, B cells typically engage antigens on the surface of antigen-presenting cells at a site termed the immunological synapse (IS). To study this important cell interaction structure, we developed a dynamic micropatterning technique that models IS formation with precise spatial and temporal control. This method enables imaging of B cells before and after B cell antigen receptor (BCR) engagement, in fixed and live samples. We analyzed BCR-proximal signaling in A20 D1.3 mouse B cells and found distinct spatial distribution preferences for different signaling proteins. Also, the size and shape of the initial non-activatory adhesion modulated the signaling outcome. We visualized the formation of the IS in living cells using enhanced-resolution microscopy in three dimensions. We identified different cell behaviors during this process, including the repurposing of pre-existing actin-based protrusions as ready-made building blocks for the IS. Overall, dynamic micropatterning provides powerful insights into the rapid, early steps of IS formation with remarkable spatial and temporal resolution.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Biomedicine and MediCity Research Laboratories, University of Turku, 20014 Turku, Finland",
    "InFLAMES Research Flagship, University of Turku, 20014 Turku, Finland",
    "Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland",
    "Department of Life Technologies, University of Turku, 20014 Turku, Finland"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Blanca Tejeda-González",
      "orcid": "0000-0001-5872-7497"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Sara Hernández-Pérez"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Elmeri Kiviluoto"
    },
    {
      "affs": [
        1,
        2,
        3
      ],
      "name": "Johanna Ivaska"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Pieta K. Mattila",
      "orcid": "0000-0003-2805-0686"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/awf492ameqkq3.bib",
  "cited_by": 1,
  "doi": "10.1242/jcs.264876",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13723395/",
  "id": "awf492ameqkq3",
  "issns": [
    "0021-9533",
    "1477-9137"
  ],
  "landing_url": "https://doi.org/10.1242/jcs.264876",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Tools and Resources"
    ],
    [
      "openalex",
      "1307|Cellular Mechanics and Interactions"
    ]
  ],
  "openalex": "W7204247717",
  "pdf_url": null,
  "pmcid": "PMC13723395",
  "pmid": "42643106",
  "published": "2026-10-22",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/awf492ameqkq3.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T12:22:02Z",
      "native_id": "PMC13723395",
      "source": "pmc",
      "url": "https://doi.org/10.1242/jcs.264876"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1307|Cellular Mechanics and Interactions",
      "subfield": "cell-biology"
    }
  ],
  "title": "Dynamic micropatterning uncovers spatial dynamics of B cell immune synapse formation",
  "title_text": "Dynamic micropatterning uncovers spatial dynamics of B cell immune synapse formation",
  "type": "peer-reviewed",
  "updated_at": "2026-11-05T12:20:13Z",
  "url": "https://research.draconitic.com/paper/awf492ameqkq3",
  "venue": "Journal of Cell Science",
  "versions": []
}