{
  "abstract": "Pediatric inflammatory bowel disease (IBD) presents more aggressively than adult-onset disease; however, epithelial pathophysiology remains poorly understood due to limited access to patient tissue. Human intestinal enteroids (HIEs) are a powerful model for investigating disease phenotypes and heterogeneity. We characterised duodenal HIEs from two cases with Crohn’s disease (ED-71 and ED-81) and one control with intestinal obstruction (ED-19), all aged <2years. Analyses included growth curves, epithelial cell heights, immunofluorescence, histology, electron microscopy, gene expression and intestinal permeability. All HIEs maintained consistent growth in vitro, with ED-81 displaying the steepest trajectory. Morphological examination revealed variations in epithelial cell height, with ED-71 and ED-81[median(95%CI): 10.2(7.76–11.4) and 12.5(11.5–15)µm] displaying a more flattened appearance than ED-19[18.7(16.8–21.0)µm, p-value:<0.001]. HIE differentiation into intestinal cell types was confirmed by gene expression and microscopy. Intestinal permeability assays indicated compromised barrier integrity in IBD-derived monolayers, with ED-81 exhibiting the highest baseline permeability (5.27%) and EGTA-induced disruption (50.03%) compared to ED-19 (3.88% and 36.82%). Pediatric intestinal enteroids in IBD demonstrate differences in epithelial growth, morphology, and barrier function. HIEs serve as a potential translational model for pediatric IBD, facilitating the study of epithelial pathophysiology and guiding precision therapy. Further studies with more pediatric HIEs are needed to confirm these findings.\n\nSupplementary Information: The online version contains supplementary material available at https://doi.org/10.1038/s41598-026-62289-6.",
  "abstract_shown": true,
  "affiliations": [
    "The Wellcome Trust Research Laboratory, Division of Gastrointestinal Sciences, Christian Medical College, Vellore, Tamil Nadu 632 004 India",
    "Centre for Stem Cell Research (CSCR), a unit of BRIC-Institute for Stem Cell Science and Regenerative Medicine (BRIC-inStem, Bengaluru), Christian Medical College, Bagayam Campus, Vellore, India",
    "Department of Gastroenterology, Division of Gastrointestinal Sciences, Christian Medical College, Vellore, India",
    "Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX USA",
    "Tufts Medical Center, Tufts University School of Medicine, Boston, MA USA"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "B. Vijayalekshmi"
    },
    {
      "affs": [
        0
      ],
      "name": "Bhuvaneswari Selvam"
    },
    {
      "affs": [
        1
      ],
      "name": "B. Sandya Rani"
    },
    {
      "affs": [
        2
      ],
      "name": "Amit K. Dutta"
    },
    {
      "affs": [
        2
      ],
      "name": "A. J. Joseph"
    },
    {
      "affs": [
        2
      ],
      "name": "Ebby Simon"
    },
    {
      "affs": [
        3
      ],
      "name": "Sasirekha Ramani"
    },
    {
      "affs": [
        3
      ],
      "name": "Mary K. Estes"
    },
    {
      "affs": [
        4
      ],
      "name": "Honorine Ward"
    },
    {
      "affs": [
        0
      ],
      "name": "Anna Pulimood"
    },
    {
      "affs": [
        0
      ],
      "name": "Sitara S. R. Ajjampur"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/axyv4kyqegetw.bib",
  "cited_by": 0,
  "doi": "10.1038/s41598-026-62289-6",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13736632/",
  "id": "axyv4kyqegetw",
  "issns": [
    "2045-2322"
  ],
  "landing_url": "https://doi.org/10.1038/s41598-026-62289-6",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "2715|Inflammatory Bowel Disease"
    ]
  ],
  "openalex": "W7172541118",
  "pdf_url": "https://www.nature.com/articles/s41598-026-62289-6_reference.pdf",
  "pmcid": "PMC13736632",
  "pmid": null,
  "published": "2026-08-05",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/axyv4kyqegetw.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:22:02Z",
      "native_id": "PMC13736632",
      "source": "pmc",
      "url": "https://doi.org/10.1038/s41598-026-62289-6"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2715|Inflammatory Bowel Disease",
      "subfield": "general"
    }
  ],
  "title": "Pediatric intestinal enteroids derived from very early onset inflammatory bowel disease reveal differences in growth, morphology, and barrier function",
  "title_text": "Pediatric intestinal enteroids derived from very early onset inflammatory bowel disease reveal differences in growth, morphology, and barrier function",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:22:02Z",
  "url": "https://research.draconitic.com/paper/axyv4kyqegetw",
  "venue": "Scientific Reports",
  "versions": []
}