{
  "abstract": "Background\n\nPolycomb group factor 1 (PCGF1) and coiled-coil domain-containing protein 34 (CCDC34) have been implicated in tumorigenesis in multiple malignancies. However, their biological functions and prognostic significance in glioma remain poorly defined.Methods\n\nRNA-sequencing data from glioma cell lines with PCGF1 knockdown were obtained from the GEO database. Correlations between PCGF1 and CCDC34 expression were analyzed using datasets from TCGA, CGGA, and GEO. In addition, RT-qPCR was performed to quantify PCGF1 and CCDC34 expression in glioma specimens. Kaplan-Meier survival analyses were conducted to evaluate the prognostic value of CCDC34. Functional assays were performed using cell lines with PCGF1 or CCDC34 knockdown and overexpression to assess their roles in glioma progression and to elucidate their regulatory relationship. Cell proliferation and colony formation assays were carried out to evaluate tumorigenic capacity.Results\n\nCCDC34 expression was significantly downregulated in glioma cell lines following PCGF1 knockdown compared with controls. CCDC34 expression positively correlated with WHO tumor grade in glioma. Elevated CCDC34 expression was significantly associated with poorer patient outcomes. Functionally, CCDC34 expression was closely linked to enhanced cell proliferation and colony-forming ability. Rescue experiments demonstrated that PCGF1 promotes glioma cell proliferation in a CCDC34-dependent manner. Gene set enrichment analysis (GSEA) further revealed that both PCGF1 and CCDC34 expression were positively associated with hypoxia, coagulation, and epithelial-mesenchymal transition (EMT) signaling pathways.Conclusion\n\nOur findings demonstrate that PCGF1 promotes glioma proliferation in a CCDC34-dependent manner and suggest that CCDC34 may serve as a novel prognostic biomarker and potential therapeutic target in glioma.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Neurosurgery, Third Xiangya Hospital, Central South University, Changsha, Hunan, China.",
    "Hunan Provincial University Key Laboratory of the Fundamental and Clinical Research on Neurodegenerative Diseases, Changsha Medical University, Changsha, Hunan, China.",
    "Department of Emergency, Xiangya Hospital, Central South University, Changsha, Hunan, China.",
    "National Clinical Research Center of Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, China.",
    "Department of Neurosurgery, The First Affiliated Hospital of University of South China, Hengyang, Hunan, China.",
    "Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China.",
    "Department of Neurosurgery, First Affiliated Hospital of Jishou University, Jishou, Hunan, China.",
    "Department of Neurosurgery, Changde Hospital, Xiangya School of Medicine, Central South University (The First People's Hospital of Changde City), Changde, Hunan, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Yuanbing Chen"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Jianbing Xiong"
    },
    {
      "affs": [
        4
      ],
      "name": "Ziran Ou"
    },
    {
      "affs": [
        5
      ],
      "name": "Tianhao Zhao"
    },
    {
      "affs": [
        5
      ],
      "name": "Hui Li"
    },
    {
      "affs": [
        5
      ],
      "name": "Jun Huang"
    },
    {
      "affs": [
        6
      ],
      "name": "Zhi Tian"
    },
    {
      "affs": [
        7
      ],
      "name": "Wuyang Cao",
      "orcid": "0009-0002-4863-1971"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ayx2cbp9aqbv9.bib",
  "cited_by": 0,
  "doi": "10.1002/acn3.70559",
  "fulltext_url": "https://europepmc.org/articles/PMC13687595",
  "id": "ayx2cbp9aqbv9",
  "issns": [
    "2328-9503"
  ],
  "landing_url": "https://europepmc.org/article/MED/42908110",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2716|Glioma Diagnosis and Treatment"
    ]
  ],
  "openalex": "W7223169659",
  "pdf_url": "https://europepmc.org/articles/PMC13687595?pdf=render",
  "pmcid": "PMC13687595",
  "pmid": "42908110",
  "published": "2026-10-21",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/ayx2cbp9aqbv9.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:53Z",
      "native_id": "MED:42908110",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42908110"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2716|Glioma Diagnosis and Treatment",
      "subfield": "human-genetics"
    }
  ],
  "title": "PCGF1 Promotes the Tumorigenesis of Malignancies Through Upregulating CCDC34 in Glioma.",
  "title_text": "PCGF1 Promotes the Tumorigenesis of Malignancies Through Upregulating CCDC34 in Glioma.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:53Z",
  "url": "https://research.draconitic.com/paper/ayx2cbp9aqbv9",
  "venue": "Annals of clinical and translational neurology",
  "versions": []
}