{
  "abstract": "The pretreatment of lignocellulosic biomass is a key step in second-generation (2G) ethanol production. In this study, sugarcane bagasse (SCB) and corn cob (CC) were pretreated with 28–30% ammonium hydroxide (1:10 w/v ratio, 30 min, 180 °C) to promote lignin removal and enhance enzymatic hydrolysis. Delignification levels reached 65.5 and 73.5% in pretreated (PT) SCB and CC, (PTSCB and PTCC, respectively), with preservation of the polysaccharide fraction. The biomasses were submitted to enzymatic saccharification using Cellic CTec3 (12.5 FPU/g cellulose) at solid concentrations of 10–30%. Fed-batch hydrolysis with 30% solids released the highest total reducing sugars (TRS), achieving 65.15 g/L in PTSCB and 70.18 g/L in PTCC, with increases of 238 and 137% in comparison to the batch at 10% of substrate. The hydrolysates presented low levels of fermentative inhibitors and high concentrations of glucose (43.75 and 41.63 g/L) and xylose (18.12 and 18.75 g/L) in PTSCB and PTCC, respectively. The fermentation of PTCC hydrolysate with the nonconventional Spathaspora passalidarum Y7204 monoculture reached the ethanol production (26.34 g/L, Yp/s = 0.44, and Qp = 0.73 g/L·h), while the commercial Saccharomyces cerevisiae Y904 yielded 19.18 g/L (Yp/s = 0.47; Qp = 0.80 g/L·h). The cocultures of both yeasts generated around 20 g/L of ethanol; however, metabolic interactions and catabolic repression may have occurred. These findings evidenced the NH4OH pretreatment as a promising strategy for enhancing saccharification. In addition, the study highlighted the positive role of S. passalidarum in the hexose (C6) and pentose (C5) cofermentation of PTCC hydrolysate.",
  "abstract_shown": true,
  "affiliations": [
    "Federal University of Uberlândia , , , ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Andressa Moreira do Nascimento"
    },
    {
      "affs": [
        0
      ],
      "name": "Gisele de Fátima Leite Souza"
    },
    {
      "affs": [
        0
      ],
      "name": "Daniel Pasquini",
      "orcid": "0000-0001-7441-5143"
    },
    {
      "affs": [
        0
      ],
      "name": "Milla Alves Baffi",
      "orcid": "0000-0003-0983-889X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/ayzzq3vfqafd5.bib",
  "cited_by": 0,
  "doi": "10.1021/acsomega.6c01259",
  "fulltext_url": "https://europepmc.org/articles/PMC13688561",
  "id": "ayzzq3vfqafd5",
  "issns": [
    "2470-1343"
  ],
  "landing_url": "https://pubs.acs.org/acsodf/article/11/41/62458/5444280/Ammonium-Hydroxide-Pretreatment-of-Sugarcane",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2204|Biofuel production and bioconversion"
    ]
  ],
  "openalex": "W7220411694",
  "pdf_url": "https://pubs.acs.org/doi/pdf/10.1021/acsomega.6c01259",
  "pmcid": "PMC13688561",
  "pmid": "42924037",
  "published": "2026-10-05",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/ayzzq3vfqafd5.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:39Z",
      "native_id": "10.1021/acsomega.6c01259",
      "source": "crossref",
      "url": "https://pubs.acs.org/acsodf/article/11/41/62458/5444280/Ammonium-Hydroxide-Pretreatment-of-Sugarcane"
    },
    {
      "first_seen": "2026-10-30T12:20:36Z",
      "native_id": "MED:42924037",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42924037"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "issn:2470-1343",
      "subfield": "general"
    }
  ],
  "title": "Ammonium Hydroxide Pretreatment of Sugarcane Bagasse and Corn Cob: Saccharification Enhancement and Ethanol Production by C6/C5 Cofermentations",
  "title_text": "Ammonium Hydroxide Pretreatment of Sugarcane Bagasse and Corn Cob: Saccharification Enhancement and Ethanol Production by C6/C5 Cofermentations",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:36Z",
  "url": "https://research.draconitic.com/paper/ayzzq3vfqafd5",
  "venue": "ACS Omega",
  "versions": []
}