{
  "abstract": "Oil red O (ORO) staining is a widely used histological technique for visualizing neutral lipids in Caenorhabditis elegans. However, quantitative image analysis of ORO-stained worms remains largely manual, time-consuming, and prone to human error. Here, we present a protocol for automating the quantification of ORO staining using Redwormpix, an open-source Python-based application. We describe steps for installing software, transferring images, and running the application. We then detail procedures for manual validation and exporting results.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "name": "Thibaud Mabit"
    },
    {
      "name": "Constance Dubois"
    },
    {
      "name": "Audrey Labarre"
    },
    {
      "name": "J. Alex Parker"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/azgk94q92q57y.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104599",
  "fulltext_url": null,
  "id": "azgk94q92q57y",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://nantes-universite.hal.science/hal-05853705v1",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "1304|Cell Image Analysis Techniques"
    ]
  ],
  "openalex": "W7162447206",
  "pdf_url": "https://nantes-universite.hal.science/hal-05853705/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-05",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/azgk94q92q57y.ris",
  "sources": [
    {
      "first_seen": "2026-11-07T12:20:25Z",
      "native_id": "hal-05853705",
      "source": "hal",
      "url": "https://nantes-universite.hal.science/hal-05853705v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1304|Cell Image Analysis Techniques",
      "subfield": "biophysics"
    }
  ],
  "title": "Protocol for automated analysis of oil red O-stained Caenorhabditis elegans using Redwormpix, a Python-based application",
  "title_text": "Protocol for automated analysis of oil red O-stained Caenorhabditis elegans using Redwormpix, a Python-based application",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T12:20:25Z",
  "url": "https://research.draconitic.com/paper/azgk94q92q57y",
  "venue": "STAR Protocols",
  "versions": []
}