{
  "abstract": "Background\n\nSound vibration is an emerging physical signal that influences plant growth and secondary metabolism; however, the genome-wide transcriptional responses of valuable medicinal orchids to complex acoustic stimuli remain largely uncharacterized.Methods\n\nHere, we performed time-series RNA-seq on acclimatized plantlets of Dendrobium huoshanense exposed to two musically structured acoustic stimuli with contrasting acoustic characteristics (Forest Fantasy and Erquan Yingyue) plus a no-music control; leaf samples were collected at 1, 6, 12, and 24 h, and quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the transcriptome data.Results\n\nThe two acoustic treatments triggered distinct transcriptional reprogramming of flavonoid biosynthesis. Early biosynthetic genes chalcone synthase (CHS) and naringenin 7-O-methyltransferase (NOMT) were rapidly up-regulated under Forest Fantasy, whereas late-step modification genes (naringenin 3-dioxygenase (F3H), flavonoid 3'-monooxygenase (F3'H), hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT), caffeoyl-CoA O-methyltransferase (CCoAOMT)) were persistently suppressed by Erquan Yingyue. Differentially expressed gene (DEG)-derived hub structural genes (cinnamate 4-hydroxylase (C4H), F3'H, CCoAOMT) and co-expressed transcription factor candidates (MYB, WRKY, NAC) were identified via Pearson correlation-based network analysis.Conclusions\n\nThese results indicate that D. huoshanense can differentiate complex musical signals at the transcript level, suggesting short-term natural-style acoustic stimulation as a promising potential horticultural strategy for quality improvement of this precious medicinal orchid.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Arts, Traditional Chinese Medicine Institute of Anhui Dabie Mountain, West Anhui University, Lu'an 237061, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Mei Huang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yinhua Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Mengru Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuxiao Ling"
    },
    {
      "affs": [
        0
      ],
      "name": "Yeyu Yin"
    },
    {
      "affs": [
        0
      ],
      "name": "Tingting Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Wei Wang",
      "orcid": "0000-0003-3100-6184"
    },
    {
      "affs": [
        0
      ],
      "name": "Shanyong Yi",
      "orcid": "0000-0001-6653-1515"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/azm2pmg2nrxtx.bib",
  "cited_by": 0,
  "doi": "10.3390/genes17101178",
  "fulltext_url": "https://europepmc.org/articles/PMC13704637",
  "id": "azm2pmg2nrxtx",
  "issns": [
    "2073-4425"
  ],
  "landing_url": "https://europepmc.org/article/MED/42938294",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Plant Gene Expression Analysis"
    ]
  ],
  "openalex": "W7214199888",
  "pdf_url": "https://europepmc.org/articles/PMC13704637?pdf=render",
  "pmcid": "PMC13704637",
  "pmid": "42938294",
  "published": "2026-09-24",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/azm2pmg2nrxtx.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:24Z",
      "native_id": "MED:42938294",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42938294"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Plant Gene Expression Analysis",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Time-Series Transcriptome Reveals Differential Regulation of Flavonoid Biosynthesis in Dendrobium huoshanense Under Musical-Acoustic Stimuli: Insights for Green Horticultural Production.",
  "title_text": "Time-Series Transcriptome Reveals Differential Regulation of Flavonoid Biosynthesis in Dendrobium huoshanense Under Musical-Acoustic Stimuli: Insights for Green Horticultural Production.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:24Z",
  "url": "https://research.draconitic.com/paper/azm2pmg2nrxtx",
  "venue": "Genes",
  "versions": []
}