{
  "abstract": "In humans, the membrane-bound serine protease prostasin encoded by Prss8 is associated with preeclampsia, a gestational hypertension disorder affecting blood supply of the placenta. Deficiency of Prss8 in mice resulted in embryonic lethality by embryonic day (E) 14.5 and impaired maturation of placental labyrinth. We found in Prss8-/- embryos at E11.5 and E12.5 a reduced frequency of both circulating primitive and erythro-myeloid progenitor (EMP)-derived definitive erythroblasts in the yolk sac, placenta and fetal liver. Single-cell RNA sequencing analyses revealed a downregulation of fetal hemoglobin genes and an upregulation of ribosomal genes in primitive erythroid cells of Prss8-/- embryos, indicating a defective erythroid maturation. We suggest that prostasin regulates primitive erythropoiesis in a cell-extrinsic manner, since its expression was absent in circulating erythroid cells. Indeed, the yolk sac vascular remodeling of Prss8-/- embryos was impaired as evidenced by reduced vessel branching and junctions, likely affecting placenta vascularization and contributing to embryonic demise. Our findings unveil a novel role for prostasin during developmental erythropoiesis and vascular patterning and provide insight into how Prss8 dysregulation may contribute to placental pathologies such as preeclampsia.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biomedical Sciences, University of Lausanne, Lausanne, Switzerland",
    "Institute of Anatomy, Stem Cell Biology, University of Zurich, Zurich, Switzerland",
    "Hematology Service, Departments of Clinical Oncology and Laboratory Medicine and Pathology, Lausanne University Hospital (CHUV) and University of Lausanne, Lausanne, Switzerland",
    "Department of Fundamental Oncology, University of Lausanne, Lausanne, Switzerland"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Sara Di Carlo"
    },
    {
      "affs": [
        1
      ],
      "name": "Adrian Salas-Bastos"
    },
    {
      "affs": [
        2
      ],
      "name": "Mariela Castelblanco Castelblanco"
    },
    {
      "affs": [
        3
      ],
      "name": "Muriel Auberson"
    },
    {
      "name": "Dongmei Wang"
    },
    {
      "name": "Marie Rumpler"
    },
    {
      "name": "Malaury Tournier"
    },
    {
      "name": "Olivier Staub"
    },
    {
      "name": "Lukas Sommer"
    },
    {
      "name": "Silvia Ferreira Lopes"
    },
    {
      "name": "Ana Jiménez Sànchez"
    },
    {
      "name": "Olaia Naveiras"
    },
    {
      "name": "Edith Hummler"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/b017rdtn3ma0e.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0360958",
  "fulltext_url": null,
  "id": "b017rdtn3ma0e",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0360958",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2729|Pregnancy and preeclampsia studies"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Cellular types/Animal cells/Blood cells/Red blood cells"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Cellular types/Animal cells/Blood cells/Red blood cells/Erythroblasts"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Cellular types/Animal cells/Bone marrow cells/Erythroblasts"
    ],
    [
      "plos",
      "/Biology and life sciences/Developmental biology/Embryology/Embryos"
    ],
    [
      "plos",
      "/Biology and life sciences/Developmental biology/Embryology/Yolk sac"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression"
    ],
    [
      "plos",
      "/Biology and life sciences/Physiology/Physiological processes/Hematopoiesis/Erythropoiesis"
    ],
    [
      "plos",
      "/Computer and information sciences/Software engineering/Computer software"
    ],
    [
      "plos",
      "/Engineering and technology/Software engineering/Computer software"
    ],
    [
      "plos",
      "/Research and analysis methods/Spectrum analysis techniques/Spectrophotometry/Cytophotometry/Flow cytometry"
    ]
  ],
  "openalex": "W7222556663",
  "pdf_url": "https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0360958&type=printable",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-16",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/b017rdtn3ma0e.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:23Z",
      "native_id": "10.1371/journal.pone.0360958",
      "source": "plos",
      "url": "https://doi.org/10.1371/journal.pone.0360958"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "plos:cell biology",
      "subfield": "cell-biology"
    },
    {
      "field": "biology",
      "origin": "plos:developmental biology",
      "subfield": "developmental-biology"
    }
  ],
  "title": "Impaired erythroid maturation in murine embryos upon loss of the preeclampsia-associated serine protease prostasin Prostasin loss impairs erythropoiesis",
  "title_text": "Impaired erythroid maturation in murine embryos upon loss of the preeclampsia-associated serine protease prostasin Prostasin loss impairs erythropoiesis",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:23Z",
  "url": "https://research.draconitic.com/paper/b017rdtn3ma0e",
  "venue": "PLOS One",
  "versions": []
}