{
  "abstract": "Surveillance of Phytophthora agathidicida , the causal agent of kauri dieback, has included soil baiting followed by loop-mediated isothermal amplification (LAMP)-based detection. This approved assay was reported to detect 1 fg of DNA using OptiGene Isothermal Master Mix on a BioRanger instrument. We evaluated its analytical robustness using the same master mix on an alternative platform (QuantStudio 3), and separately tested an alternative NEB master mix. Both assays yielded a reliable detection limit of 1,000 fg, with variable detection at 100 fg and none below. Diagnostic laboratories should verify assay performance under their operational conditions prior to implementation.",
  "abstract_shown": true,
  "affiliations": [
    "School of Biological Sciences, Victoria University of Wellington, New Zealand."
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jade Tt Palmer"
    },
    {
      "affs": [
        0
      ],
      "name": "Monica L Gerth"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/b08ns58bq4rwj.bib",
  "cited_by": 0,
  "doi": "10.17912/micropub.biology.002163",
  "fulltext_url": "https://europepmc.org/articles/PMC13659447",
  "id": "b08ns58bq4rwj",
  "issns": [
    "2578-9430"
  ],
  "landing_url": "https://europepmc.org/article/MED/42871916",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1110|Plant Pathogens and Resistance"
    ]
  ],
  "openalex": "W7214704484",
  "pdf_url": "https://europepmc.org/articles/PMC13659447?pdf=render",
  "pmcid": "PMC13659447",
  "pmid": "42871916",
  "published": "2026-09-29",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/b08ns58bq4rwj.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:16Z",
      "native_id": "MED:42871916",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42871916"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1110|Plant Pathogens and Resistance",
      "subfield": "plant-biology"
    }
  ],
  "title": "The limit of detection of a Phytophthora agathidicida LAMP assay is higher under alternative laboratory conditions.",
  "title_text": "The limit of detection of a Phytophthora agathidicida LAMP assay is higher under alternative laboratory conditions.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:16Z",
  "url": "https://research.draconitic.com/paper/b08ns58bq4rwj",
  "venue": "MicroPublication biology",
  "versions": []
}