{
  "abstract": "Human embryonic development proceeds more slowly than in mice. The segmentation clock offers a tractable model for studying species-specific developmental tempo, as its oscillation period in human induced presomitic mesoderm (iPSM) cells is approximately twice that of mouse. While the core clock gene HES7 exhibits slower protein degradation in human cells, it remains unclear whether such cross-species differences in protein stability reflect a general principle. Here, we perform a dynamic stable isotope labeling of amino acids in cell culture (SILAC)-based proteomic analysis of ∼5,000 proteins in human and mouse iPSM, and we uncover a broad trend of slower protein degradation in human cells, regardless of subcellular localization or degradation pathways. Moreover, inhibition of glycolysis in mouse iPSM partially phenocopies the human protein stability profile, and modulation of protein stability alters the tempo of both the segmentation clock and cellular differentiation. Our findings establish protein stability, with systematic differences across species, as a key mediator linking metabolism to developmental tempo.",
  "abstract_shown": true,
  "affiliations": [
    "European Molecular Biology Laboratory, EMBL Barcelona, C/ Dr. Aiguader, 88, PRBB Building, 08003 Barcelona, Spain; Cluster of Excellence Physics of Life, TU Dresden, 01307 Dresden, Germany",
    "European Molecular Biology Laboratory, Genome Biology Unit, 69117 Heidelberg, Germany",
    "European Molecular Biology Laboratory, EMBL Barcelona, C/ Dr. Aiguader, 88, PRBB Building, 08003 Barcelona, Spain; Collaboration for joint PhD degree between EMBL and Heidelberg University, Faculty of Biosciences, Heidelberg, Germany",
    "European Molecular Biology Laboratory, Genome Biology Unit, 69117 Heidelberg, Germany. Electronic address: mikhail.savitski@embl.de",
    "European Molecular Biology Laboratory, EMBL Barcelona, C/ Dr. Aiguader, 88, PRBB Building, 08003 Barcelona, Spain; Cluster of Excellence Physics of Life, TU Dresden, 01307 Dresden, Germany; Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany. Electronic address: miki.ebisuya@tu-dresden.de"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Mitsuhiro Matsuda",
      "orcid": "0000-0003-1592-5199"
    },
    {
      "affs": [
        1
      ],
      "name": "Henrik M. Hammarén",
      "orcid": "0000-0002-8534-2530"
    },
    {
      "affs": [
        2
      ],
      "name": "Jorge Lázaro",
      "orcid": "0000-0002-9901-356X"
    },
    {
      "affs": [
        3
      ],
      "name": "Mikhail M. Savitski",
      "orcid": "0000-0003-2011-9247"
    },
    {
      "affs": [
        4
      ],
      "name": "Miki Ebisuya",
      "orcid": "0000-0003-1854-7496"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/b1hm6y4n1zrz6.bib",
  "cited_by": 10,
  "doi": "10.1016/j.devcel.2026.07.012",
  "fulltext_url": null,
  "id": "b1hm6y4n1zrz6",
  "issns": [
    "1534-5807",
    "1878-1551"
  ],
  "landing_url": "https://doi.org/10.1016/j.devcel.2026.07.012",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Developmental Biology and Gene Regulation"
    ]
  ],
  "openalex": "W7202343776",
  "pdf_url": "https://www.sciencedirect.com/science/article/pii/S1534580726002819/pdf",
  "pmcid": null,
  "pmid": "42594862",
  "published": "2026-08-13",
  "publisher": "Elsevier BV",
  "ris": "https://research.draconitic.com/paper/b1hm6y4n1zrz6.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:26:46Z",
      "native_id": "W7202343776",
      "source": "openalex",
      "url": "https://doi.org/10.1016/j.devcel.2026.07.012"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Developmental Biology and Gene Regulation",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Systematic differences in protein stability underlie species-specific developmental tempo",
  "title_text": "Systematic differences in protein stability underlie species-specific developmental tempo",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T13:21:07Z",
  "url": "https://research.draconitic.com/paper/b1hm6y4n1zrz6",
  "venue": "Developmental Cell",
  "versions": []
}