{
  "abstract": "Objective: The present study aimed to investigate post-thaw semen quality and sperm kinematics between Peranakan Etawah (PE) and Saanen goats, and to identify breed-associated proteomic markers related to semen cryotolerance.\n\nMethods: Frozen semen straws were obtained from BIB Singosari, Indonesia, and grouped by breed. Post-thaw evaluations included cellular viability, morphology/abnormality, plasma membrane integrity (HOST), and computer-assisted sperm analysis for motility and kinematic parameters. Sperm proteomes were profiled via UHPLC–Orbitrap LC–MS/MS. Data were processed in Proteome Discoverer and analyzed using multivariate models (principal component analysis [PCA], partial least squares–discriminant analysis [PLS-DA]), differential abundance testing (q-values), functional enrichment (g:Profiler), biomarker selection (variable importance in projection>1; area under the curve≥0.70), and correlation with semen traits (MetaboAnalyst).\n\nResults: PE and Saanen differed significantly in survival-related traits, including viability, abnormalities, and plasma membrane integrity (p<0.05), while total motility and other kinematic parameters were similar between breeds (p>0.05). Proteomic patterns showed partial separation by PCA and more distinct differentiation through verified PLS-DA. Multiple proteins exhibited variable abundance between the breeds (q<0.05) and clustered reliably by breed. Enrichment analysis highlighted mitochondrion-associated functions, particularly fatty acid β-oxidation. Identified discriminative proteins included ACADVL, ETFB, GPD2, CYLC1, and LOC102188264, which were significantly associated with semen quality traits within breeds.\n\nConclusion: Differences across breeds in frozen–thawed goat semen were mostly evident in post-thaw viability traits and in proteomic signatures predominantly influenced by mitochondrial energy metabolism. Integrating proteomics with conventional semen assessment may elucidate semen cryotolerance and support biomarker identification.",
  "abstract_shown": true,
  "affiliations": [
    "Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia",
    "Research Center for Animal Husbandry, Research Organization for Agriculture and Food, Bogor, Indonesia",
    "Research Center for Applied Zoology, National Research and Innovation Agency, Bogor, Indonesia",
    "Graduate Student of Animal Production and Technology Science, Graduate School, IPB University, Bogor, Indonesia",
    "Graduate Student of Animal Biomedical Science Study Program, School of Veterinary Medicine and Biomedical Science, IPB University, Bogor, Indonesia"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Muhammad Ihsan Andi Dagong",
      "orcid": "0000-0001-5060-2614"
    },
    {
      "affs": [
        0
      ],
      "name": "Muhammad Yusuf",
      "orcid": "0000-0003-3421-4384"
    },
    {
      "affs": [
        0
      ],
      "name": "Athhar Manabi Diansyah",
      "orcid": "0009-0002-2338-8215"
    },
    {
      "affs": [
        1
      ],
      "name": "Procula Rudlof Matitaputty",
      "orcid": "0000-0003-3376-2266"
    },
    {
      "affs": [
        2
      ],
      "name": "Paskah Partogi Agung",
      "orcid": "0000-0003-2554-1893"
    },
    {
      "affs": [
        3
      ],
      "name": "Indra Wahyudi Syarif",
      "orcid": "0009-0001-7774-7896"
    },
    {
      "affs": [
        2,
        4
      ],
      "name": "Muhammad Fajar Amrullah",
      "orcid": "0000-0003-1589-4572"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/b1s39ryfxpqne.bib",
  "cited_by": 0,
  "doi": "10.5713/ab.260268",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13740641/",
  "id": "b1s39ryfxpqne",
  "issns": [
    "2765-0189",
    "2765-0235"
  ],
  "landing_url": "https://doi.org/10.5713/ab.260268",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Animal Reproduction and Physiology"
    ],
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "2743|Sperm and Testicular Function"
    ]
  ],
  "openalex": "W7171366158",
  "pdf_url": "https://www.animbiosci.org/upload/pdf/ab-260268.pdf",
  "pmcid": "PMC13740641",
  "pmid": "42526860",
  "published": "2026-07-27",
  "publisher": "Asian-Australasian Association of Animal Production Societies (AAAP)",
  "ris": "https://research.draconitic.com/paper/b1s39ryfxpqne.ris",
  "sources": [
    {
      "first_seen": "2026-11-10T12:21:51Z",
      "native_id": "PMC13740641",
      "source": "pmc",
      "url": "https://doi.org/10.5713/ab.260268"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2743|Sperm and Testicular Function",
      "subfield": "reproductive-child"
    }
  ],
  "title": "Putative proteomic signatures associated with post-thaw semen quality in Peranakan Etawah and Saanen goats: an exploratory study",
  "title_text": "Putative proteomic signatures associated with post-thaw semen quality in Peranakan Etawah and Saanen goats: an exploratory study",
  "type": "peer-reviewed",
  "updated_at": "2026-11-10T12:21:51Z",
  "url": "https://research.draconitic.com/paper/b1s39ryfxpqne",
  "venue": "Animal Bioscience",
  "versions": []
}