{
  "abstract": "Objective\n\nAlthough synovial inflammation is recognized as a factor in the progression of osteoarthritis (OA), the specific role of fibroblast-like synoviocytes (FLS) in cartilage degradation has yet to be fully elucidated. This study sought to identify key regulators that connect inflammatory FLS to chondrocyte dysfunction.Methods\n\nAn integrated re-analysis of bulk and single-cell RNA sequencing data from OA synovial tissues was conducted to identify consistently upregulated genes. Functional assays, including migration and invasion tests, were performed on inflammatory FLS. FLS with silenced CYBB expression were co-cultured with chondrocytes to evaluate paracrine effects. small extracellular vesicle (sEV) isolation and tracking were utilized to assess intercellular communication. An OA rat model was employed for in vivo validation through local CYBB knockdown.Results\n\nCYBB expression was markedly upregulated in OA synovium, particularly within inflammatory FLS. Inhibition of CYBB reduced FLS inflammation, migration, and invasion. In co-culture experiments, inflammatory FLS induced chondrocyte inflammation and disrupted matrix gene expression in a manner dependent on CYBB. These effects were mediated by sEVs derived from fibroblast-like synoviocytes (FLS), with their inflammatory cargo being modified through CYBB silencing. In vivo experiments demonstrated that localized suppression of CYBB alleviated synovitis and cartilage degeneration.Conclusions\n\nCYBB serves as a pivotal mediator linking synovial inflammation to cartilage damage in osteoarthritis (OA), by mechanistically regulating the sEV-mediated communication from FLS to chondrocytes. Targeting CYBB could potentially represent a novel therapeutic strategy for OA.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Orthopedics / Central Laboratory, Affiliated Hai'an Hospital of Nantong University, Zhongba Road 17, Hai'an, Jiangsu, 226600, China.",
    "Department of Traumatology and Orthopedics, The Third Affiliated Hospital of Soochow University, The First People's Hospital of Changzhou City, Juqian Road 185, Changzhou City, 213000, China.",
    "Department of Orthopedics, The First Affiliated Hospital of Soochow University, Shizi Street 188, Suzhou, Jiangsu, 215000, China.",
    "Department of Pathology, Affiliated Hai'an Hospital of Nantong University, Zhongba Road 17, Hai'an, Jiangsu, 226600, China.",
    "Department of Orthopedics, the Affiliated Suzhou Hospital of Nanjing Medical University, Guangji Road 242, Suzhou, Jiangsu, 215000, China.",
    "Department of Orthopedics, The First Affiliated Hospital of Soochow University, Shizi Street 188, Suzhou, Jiangsu, 215000, China. szgengdc@suda.edu.cn.",
    "Department of Traumatology and Orthopedics, The Third Affiliated Hospital of Soochow University, The First People's Hospital of Changzhou City, Juqian Road 185, Changzhou City, 213000, China. flying_ding@126.com.",
    "Department of Orthopedics / Central Laboratory, Affiliated Hai'an Hospital of Nantong University, Zhongba Road 17, Hai'an, Jiangsu, 226600, China. shujunlv@163.com."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Kai Chen"
    },
    {
      "affs": [
        1
      ],
      "name": "Xiaoyu Dai"
    },
    {
      "affs": [
        2
      ],
      "name": "Peizhen Wang"
    },
    {
      "affs": [
        3
      ],
      "name": "Chuyi Wang"
    },
    {
      "affs": [
        2
      ],
      "name": "Chang Lv"
    },
    {
      "affs": [
        4
      ],
      "name": "Huaqiang Tao"
    },
    {
      "affs": [
        5
      ],
      "name": "Dechun Geng"
    },
    {
      "affs": [
        6
      ],
      "name": "Wenge Ding"
    },
    {
      "affs": [
        7
      ],
      "name": "Shujun Lv"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/b383zx9d8b40w.bib",
  "cited_by": 0,
  "doi": "10.1186/s13075-026-03866-y",
  "fulltext_url": "https://europepmc.org/articles/PMC13679134",
  "id": "b383zx9d8b40w",
  "issns": [
    "1478-6354",
    "1478-6362"
  ],
  "landing_url": "https://europepmc.org/article/MED/42894224",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "RC925-935"
    ],
    [
      "openalex",
      "2745|Osteoarthritis Treatment and Mechanisms"
    ]
  ],
  "openalex": "W7170167581",
  "pdf_url": "https://europepmc.org/articles/PMC13679134?pdf=render",
  "pmcid": "PMC13679134",
  "pmid": "42894224",
  "published": "2026-07-01",
  "publisher": "BMC",
  "ris": "https://research.draconitic.com/paper/b383zx9d8b40w.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:42Z",
      "native_id": "MED:42894224",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42894224"
    },
    {
      "first_seen": "2026-10-30T13:46:10Z",
      "native_id": "771b0d2a5f3741b98f2510ffc0c10d93",
      "source": "doaj",
      "url": "https://doi.org/10.1186/s13075-026-03866-y"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2745|Osteoarthritis Treatment and Mechanisms",
      "subfield": "general"
    }
  ],
  "title": "CYBB regulates synovial fibroblast inflammatory activation and sEV-mediated synovial-cartilage communication in osteoarthritis.",
  "title_text": "CYBB regulates synovial fibroblast inflammatory activation and sEV-mediated synovial-cartilage communication in osteoarthritis.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:46:10Z",
  "url": "https://research.draconitic.com/paper/b383zx9d8b40w",
  "venue": "Arthritis research & therapy",
  "versions": []
}