{
  "abstract": "Post-translational modifications (PTMs) are critical to protein activity, yet the biological role of most known modification sites remains uncharacterized. CRISPR-mediated phenotypic screens using base editors offer a powerful approach to dissecting PTM function at scale. However, existing single-guide RNA (sgRNA) design tools for base editing applications are DNA-centric and lack the throughput required to integrate seamlessly with mass-spectrometry-based proteomics experimental outputs. We introduce protein editing in R, PrEditR, an open-source, protein-centric tool for high-throughput sgRNA design for custom base editor screens. PrEditR enables users to designate specific amino acid residues in proteins and design protospacer sequences that target the corresponding gene and annotate every resulting amino acid change (missense, nonsense, or silent), including bystander edits. PrEditR is available on GitHub and Docker Hub.",
  "abstract_shown": true,
  "affiliations": [
    "La Jolla Institute for Immunology, Laboratory for Immunochemical Circuits, La Jolla, CA 92037, United States.",
    "Bioinformatics and Systems Biology Ph.D. Program, University of California San Diego, La Jolla, CA 92093, United States.",
    "Center of Autoimmunity and Inflammation, La Jolla Institute for Immunology, La Jolla, CA 92037, United States.",
    "Department of Pharmacology, University of California San Diego, La Jolla, CA 92037, United States.",
    "Moores Cancer Center, UCSD Health, La Jolla, CA 92037, United States."
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Felipe Vásquez-Castro",
      "orcid": "0000-0003-2591-7117"
    },
    {
      "affs": [
        0
      ],
      "name": "Leonardo D Sanchez Solis",
      "orcid": "0000-0003-4492-8305"
    },
    {
      "affs": [
        0,
        2,
        3,
        4
      ],
      "name": "Samuel A Myers",
      "orcid": "0000-0001-7636-0887"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/b3w47q3mysb72.bib",
  "cited_by": 0,
  "doi": "10.1093/bioadv/vbag323",
  "fulltext_url": "https://europepmc.org/articles/PMC13744405",
  "id": "b3w47q3mysb72",
  "issns": [
    "2635-0041"
  ],
  "landing_url": "https://europepmc.org/article/MED/42999912",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|CRISPR and Genetic Engineering"
    ]
  ],
  "openalex": "W7161416794",
  "pdf_url": "https://europepmc.org/articles/PMC13744405?pdf=render",
  "pmcid": "PMC13744405",
  "pmid": "42999912",
  "published": "2026-10-30",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/b3w47q3mysb72.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T15:20:34Z",
      "native_id": "MED:42999912",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42999912"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|CRISPR and Genetic Engineering",
      "subfield": "molecular-biology"
    }
  ],
  "title": "PrEditR: a protein-centric platform for CRISPR-mediated base editor sgRNA design.",
  "title_text": "PrEditR: a protein-centric platform for CRISPR-mediated base editor sgRNA design.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-10T03:20:49Z",
  "url": "https://research.draconitic.com/paper/b3w47q3mysb72",
  "venue": "Bioinformatics advances",
  "versions": []
}