{
  "abstract": "This study evaluated the susceptibility to lung and kidney carcinogenesis induced by N-bis(2-hydroxypropyl)nitrosamine (DHPN) in female rats whose dams received a low-protein diet (LPD) during gestation. Pregnant Wistar rats were fed normal protein diet (NPD, 17% protein) or LPD (6% protein). After parturition, all dams were fed NPD until weaning. Then, female offspring were distributed into four groups (NPD, LPD, NPD + DHPN, and LPD + DHPN) and fed NPD. At 6 weeks old, two groups received 0.2% DHPN in drinking water for 4 weeks, and some females were euthanized 24 h after carcinogen stopping, while others were euthanized at Week 26. First, the lung was removed, fixed, histologically processed, and tissue sections were immunohistochemically stained for γ-H2AX. Second, the lung and kidneys were removed, fixed, histologically processed, and hematoxylin-eosin stained tissue sections were obtained for histopathological analysis. Female rats whose dams received LPD developed a higher mean number of pulmonary epithelial cells positive for γ-H2AX (p < 0.005) and bronchioloalveolar hyperplasia and adenomas (p < 0.001 and p < 0.001) in the lung compared with their counterparts. LPD did not alter the development of atypical tubule hyperplasia in the kidney. These findings indicated that LPD increased DHPN-induced lung carcinogenesis in female rats.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Cellular and Molecular Biology, Institute of Biosciences, UNESP-São Paulo State University, Botucatu, SP, Brazil.",
    "Department of Internal Medicine, Nucleus of Medicine and Experimental Surgery, FCM, UNICAMP-State University of Campinas, Campinas, SP, Brazil."
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Vanessa de Cássia Martins Pires Ferreira",
      "orcid": "0000-0001-9921-7821"
    },
    {
      "affs": [
        0
      ],
      "name": "Victor Hugo Carvalho"
    },
    {
      "affs": [
        1
      ],
      "name": "Patrícia Aline Boer",
      "orcid": "0000-0002-1196-2606"
    },
    {
      "affs": [
        0
      ],
      "name": "Luís Fernando Barbisan",
      "orcid": "0000-0002-2180-1814"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/b3z555jfhwrag.bib",
  "cited_by": 0,
  "doi": "10.1002/em.70086",
  "fulltext_url": "https://europepmc.org/articles/PMC13695019",
  "id": "b3z555jfhwrag",
  "issns": [
    "0893-6692",
    "1098-2280"
  ],
  "landing_url": "https://europepmc.org/article/MED/42920348",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1306|Carcinogens and Genotoxicity Assessment"
    ]
  ],
  "openalex": "W7223468053",
  "pdf_url": "https://europepmc.org/articles/PMC13695019?pdf=render",
  "pmcid": "PMC13695019",
  "pmid": "42920348",
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/b3z555jfhwrag.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:36Z",
      "native_id": "MED:42920348",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42920348"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1306|Carcinogens and Genotoxicity Assessment",
      "subfield": "oncology"
    }
  ],
  "title": "Gestational Low Protein Diet Increases the Susceptibility to N-Bis(2-Hydroxypropyl)nitrosamine-Induced Lung Tumors in Female Wistar Rats.",
  "title_text": "Gestational Low Protein Diet Increases the Susceptibility to N-Bis(2-Hydroxypropyl)nitrosamine-Induced Lung Tumors in Female Wistar Rats.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:36Z",
  "url": "https://research.draconitic.com/paper/b3z555jfhwrag",
  "venue": "Environmental and molecular mutagenesis",
  "versions": []
}