{
  "abstract": "Radiation-induced skeletal deterioration and bone loss remain major clinical challenges. Although exosomes derived from human bone marrow-derived mesenchymal stromal cells (hBMSCs) (MSC-Exos) are promising cell-free therapeutics, their limited stability and low delivery efficiency hinder translational application. Here, we developed an innovative microdroplet-based exosome delivery platform (MD/Exos) and systematically evaluated its protective effects under irradiation-induced stress. MSC-Exos were successfully isolated and characterized using transmission electron microscopy and western blot analysis. Fluorescence imaging and confocal microscopy confirmed efficient uptake of MSC-Exos by both macrophages and hBMSCs. MD/Exos were fabricated by encapsulating exosomes within bovine serum albumin-stabilized microdroplets. Cytocompatibility assays showed that MD/Exos exhibited no detectable cytotoxicity toward macrophages. Functionally, MD/Exos significantly enhanced the viability, metabolic activity, and cytoskeletal integrity of irradiated hBMSCs. Notably, MD/Exos markedly promoted osteogenic differentiation after irradiation, as evidenced by increased alkaline phosphatase activity, enhanced mineralized matrix deposition, and upregulated osteogenesis-related gene expression. In parallel, MD/Exos modulated macrophage responses to radiation by suppressing aberrant multinucleated giant-cell formation and reducing pro-inflammatory cytokine expression. In vivo, administration of MD/Exos in an irradiation-induced bone injury model significantly attenuated bone loss and promoted bone regeneration, as evidenced by preserved bone microstructure, reduced osteoclast activity, and downregulated tumor necrosis factor-alpha (TNF-α) expression. Further in vivo cytokine analysis indicated that MD/Exos treatment significantly reduced pro-inflammatory cytokines compared to irradiated controls. Collectively, these findings demonstrate that MD/Exos represent a biocompatible and efficient exosome delivery platform capable of mitigating irradiation-induced cellular dysfunction, enhancing osteogenesis, and modulating immune-bone crosstalk. This study highlights the therapeutic potential of MD/Exos as a novel strategy for the prevention and treatment of radiation-induced bone loss.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Orthopaedic Surgery, The Eighth Affiliated Hospital of Southern Medical University (The First People's Hospital of Shunde), Foshan, Guangdong, 528308, China.",
    "Department of Epidemiology and Health Statistics, School of Public Health, Zunyi Medical University, Zunyi, Guizhou, 563000, China.",
    "Department of Orthopaedic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, 563003, China.",
    "Department of Orthopaedic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, 563003, China. 506828564@qq.com.",
    "Department of Orthopaedic Surgery, The Eighth Affiliated Hospital of Southern Medical University (The First People's Hospital of Shunde), Foshan, Guangdong, 528308, China. xinwang56@163.com.",
    "Department of Orthopaedic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, 563003, China. xinwang56@163.com.",
    "Zunyi Municipal Key Laboratory of Medicinal Biotechnology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, 563003, China. xinwang56@163.com.",
    "Institute of Medicinal Biotechnology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, 563003, China. xinwang56@163.com."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Yi Zhang",
      "orcid": "0000-0003-2933-1818"
    },
    {
      "affs": [
        2
      ],
      "name": "Wenqing Huang"
    },
    {
      "affs": [
        2
      ],
      "name": "Xianglin Peng"
    },
    {
      "affs": [
        2
      ],
      "name": "Haiping Lu"
    },
    {
      "affs": [
        2
      ],
      "name": "Yaping Ma"
    },
    {
      "affs": [
        3
      ],
      "name": "Ansu Wang"
    },
    {
      "affs": [
        4,
        5,
        6,
        7
      ],
      "name": "Xin Wang",
      "orcid": "0000-0001-5015-3396"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/b433yshymn5ng.bib",
  "cited_by": 0,
  "doi": "10.1186/s13036-026-00748-4",
  "fulltext_url": "https://europepmc.org/articles/PMC13729329",
  "id": "b433yshymn5ng",
  "issns": [
    "1754-1611"
  ],
  "landing_url": "https://europepmc.org/article/MED/42972386",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research"
    ],
    [
      "openalex",
      "1312|Extracellular vesicles in disease"
    ]
  ],
  "openalex": "W7196998792",
  "pdf_url": "https://europepmc.org/articles/PMC13729329?pdf=render",
  "pmcid": "PMC13729329",
  "pmid": "42972386",
  "published": "2026-08-06",
  "publisher": "BMC",
  "ris": "https://research.draconitic.com/paper/b433yshymn5ng.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T15:20:49Z",
      "native_id": "MED:42972386",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42972386"
    },
    {
      "first_seen": "2026-11-07T12:22:06Z",
      "native_id": "PMC13729329",
      "source": "pmc",
      "url": "https://doi.org/10.1186/s13036-026-00748-4"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Extracellular vesicles in disease",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Microdroplet-mediated exosome delivery system for the therapeutic management of radiation-induced bone damage.",
  "title_text": "Microdroplet-mediated exosome delivery system for the therapeutic management of radiation-induced bone damage.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T12:22:06Z",
  "url": "https://research.draconitic.com/paper/b433yshymn5ng",
  "venue": "Journal of biological engineering",
  "versions": []
}