{
  "abstract": "Microplastic pollution has become a growing global concern, and fungal biodegradation represents a promising approach for its mitigation. Accurate quantification of adipic acid (AA), a key monomer released during the degradation of poly(1,4-butylene adipate)-based polyurethane (PBA-PU), is essential for confirming the release of adipic acid units during PBA-PU degradation and verifying ester bond hydrolysis. A rapid and straightforward method was developed and validated using high-performance liquid chromatography - high resolution mass spectrometry (LC-HRMS) for AA quantification. The method, which is derivatization-free, matrix-resistant, and cost-effective, enables sensitive and precise trace-level detection of AA in complex fungal degradation matrices. It involves the addition of adipic acid- d 8 (deuterium-labeled AA) as an internal standard, followed by extraction through filtration and methanol dissolution. The extracts were analyzed on a reverse-phase C-18 column using liquid chromatography. The optimized analytical method enables accurate, sensitive, and efficient AA quantification within 6 min, demonstrating excellent linearity (R 2 > 0.99), matrix effect (-0.519%), precision (RSD < 10%), recovery (89.32 - 104.19%), stability (RSD < 15%), and limits of quantification (LOQ) of 15 ng/mL. This method was successfully applied to analyze AA produced during the fungal degradation of PBA-PU by Cladosporium oxysporum SCSIO 81042 (a marine-derived fungus), highlighting its applicability for routine analysis of AA monomers quantitative detection in microbial plastic degradation.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Biomedical Health Technology and Engineering, Shenzhen Bay Laboratory, Shenzhen, 518132, China",
    "State Key Laboratory of Chemical Oncogenomics, Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China",
    "State Key Laboratory of Chemical Oncogenomics, Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China; School of Chemistry, South China Normal University, Guangzhou, 510006, China",
    "Institute of Biomedical Health Technology and Engineering, Shenzhen Bay Laboratory, Shenzhen, 518132, China; Corresponding author."
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Zhichao Shi"
    },
    {
      "affs": [
        1
      ],
      "name": "Lihong Wu"
    },
    {
      "affs": [
        2
      ],
      "name": "Hui Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Tingbiao Wu"
    },
    {
      "affs": [
        0
      ],
      "name": "Yingjuan Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Boren Xiao"
    },
    {
      "affs": [
        3
      ],
      "name": "Tingting Fan"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/b4xsvkmc9fsc7.bib",
  "cited_by": 0,
  "doi": "10.1016/j.jcoa.2026.100372",
  "fulltext_url": "http://www.sciencedirect.com/science/article/pii/S2772391726000666",
  "id": "b4xsvkmc9fsc7",
  "issns": [
    "2772-3917"
  ],
  "landing_url": "https://doi.org/10.1016/j.jcoa.2026.100372",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "QD71-142"
    ],
    [
      "openalex",
      "2310|Microplastics and Plastic Pollution"
    ]
  ],
  "openalex": "W7221804394",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-30",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/b4xsvkmc9fsc7.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:44:27Z",
      "native_id": "0205952feed44b47a40e622a15f48630",
      "source": "doaj",
      "url": "https://doi.org/10.1016/j.jcoa.2026.100372"
    }
  ],
  "subjects": [
    {
      "field": "earth",
      "origin": "openalex:2310|Microplastics and Plastic Pollution",
      "subfield": "environmental-science"
    }
  ],
  "title": "A high-sensitivity and matrix-resistant liquid chromatography - high resolution mass spectrometry method for accurate quantification of adipic acid in fungal microplastic degradation systems",
  "title_text": "A high-sensitivity and matrix-resistant liquid chromatography - high resolution mass spectrometry method for accurate quantification of adipic acid in fungal microplastic degradation systems",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T14:20:20Z",
  "url": "https://research.draconitic.com/paper/b4xsvkmc9fsc7",
  "venue": "Journal of Chromatography Open",
  "versions": []
}