{
  "abstract": "Phytolacca americana L. (Phytolaccaceae), a perennial species native to North America, is rich in oleanane-type triterpenoid saponins. In this study, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC–QTOF–MS) profiling of the aerial-part extract revealed characteristic fragment ions of phytolaccagenin- and phytolaccagenic acid-type saponins with diverse glycosylation. MS-guided annotation enabled targeted isolation and structural elucidation of two previously undescribed compounds, one oleanane-type triterpenoid saponin (1) and one neolignan (3). Additionally, compound 2, which had been reported only based on mass spectrometric data, was isolated, and its structure was fully elucidated for the first time, together with those of 10 known oleanane-type triterpenoid saponins (4–13). Their structures were elucidated by comprehensive one-dimensional and two-dimensional nuclear magnetic resonance spectroscopic analyses combined with UPLC–QTOF–MS data. Although P. americana extracts have been reported to inhibit influenza A virus infection in previous studies, the bioactive constituents responsible for this antiviral activity have not been identified. The crude extract and isolated saponins were evaluated using complementary in vitro assays. In H1N1-infected A549 cells, compound 11 (3-O-trisaccharide phytolaccagenic acid) reduced viral NS1 RNA levels at 6 and 24 hpi as determined by RT–qPCR. Antiviral activity was further confirmed by plaque reduction and immunoblot analyses, with a plaque-assay IC50 of 57.98 ± 0.26 µM.",
  "abstract_shown": true,
  "affiliations": [
    "College of Pharmacy, Chungnam National University 1 , Daejeon, Republic of Korea",
    "Department of Life Science and Multidisciplinary Genome Institute, Hallym University 2 , Chuncheon, Republic of Korea",
    "Hallym AI-Biohealth R&BD Center, Hallym University 3 , Chuncheon, Republic of Korea"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Shuo Tian"
    },
    {
      "affs": [
        0
      ],
      "name": "Jisu Park"
    },
    {
      "affs": [
        1
      ],
      "name": "You Jin Kim"
    },
    {
      "affs": [
        0
      ],
      "name": "Sainan Li"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Wonkyun Ronny Im"
    },
    {
      "affs": [
        1
      ],
      "name": "Sung Ho Jeon"
    },
    {
      "affs": [
        0
      ],
      "name": "MinKyun Na",
      "orcid": "0000-0002-4865-6506"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/b53z6p987me9d.bib",
  "cited_by": 0,
  "doi": "10.1098/rsos.262034",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13730658/?tool=EBI",
  "id": "b53z6p987me9d",
  "issns": [
    "2054-5703"
  ],
  "landing_url": "https://royalsocietypublishing.org/rsos/article/13/11/262034/484012/Triterpenoid-saponins-from-the-aerial-parts-of",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Chemical Biology"
    ],
    [
      "heading",
      "Chemistry"
    ],
    [
      "heading",
      "Medicinal Chemistry"
    ],
    [
      "heading",
      "Organic Chemistry"
    ],
    [
      "heading",
      "Research"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://royalsocietypublishing.org/rsos/article-pdf/doi/10.1098/rsos.262034/6175261/rsos.262034.pdf",
  "pmcid": "PMC13730658",
  "pmid": "42979405",
  "published": "2026-11-04",
  "publisher": "The Royal Society",
  "ris": "https://research.draconitic.com/paper/b53z6p987me9d.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T12:20:47Z",
      "native_id": "10.1098/rsos.262034",
      "source": "crossref",
      "url": "https://royalsocietypublishing.org/rsos/article/13/11/262034/484012/Triterpenoid-saponins-from-the-aerial-parts-of"
    },
    {
      "first_seen": "2026-11-04T15:20:46Z",
      "native_id": "PMC:PMC13730658",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13730658"
    },
    {
      "first_seen": "2026-11-07T12:23:10Z",
      "native_id": "PMC13730658",
      "source": "pmc",
      "url": "https://doi.org/10.1098/rsos.262034"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "heading:Chemical Biology",
      "subfield": "chemical-biology"
    },
    {
      "field": "chemistry",
      "origin": "heading:Medicinal Chemistry",
      "subfield": "medicinal"
    },
    {
      "field": "chemistry",
      "origin": "heading:Organic Chemistry",
      "subfield": "organic"
    }
  ],
  "title": "Triterpenoid saponins from the aerial parts of Phytolacca americana and their anti-influenza A virus activity",
  "title_text": "Triterpenoid saponins from the aerial parts of Phytolacca americana and their anti-influenza A virus activity",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T12:23:10Z",
  "url": "https://research.draconitic.com/paper/b53z6p987me9d",
  "venue": "Royal Society Open Science",
  "versions": []
}