{
  "abstract": "The escalating crisis of antimicrobial resistance, particularly among Gram-negative bacteria, demands innovative therapeutic strategies. Here, we present a dual-targeting lipid nanoparticle-chimeric phage (AbLCP) system engineered to combat multidrug-resistant (MDR) Gram-negative bacteria. Our design integrates a genetically modified M13 phage displaying anti-lipopolysaccharide (LPS) antibodies with polymyxin B conjugation, which is further encapsulated within anti-LPS antibody-functionalized lipid nanoparticles. This system overcomes the fundamental limitations of conventional phage therapy, enabling efficient cellular internalization, endosomal escape, and potent intracellular bactericidal activity. In murine models of Pseudomonas aeruginosa infection, AbLCP achieved superior bacterial clearance and up to 10³-fold reduction in bacterial burden at a 10³-fold lower effective dose than free polymyxin B, with 67-fold selective accumulation at infection sites, and attenuated systemic inflammation and preservation of gut microbiota. Mechanistically, AbLCP neutralized LPS to suppress inflammation and induced a lethal bacterial stress response. This work establishes a transformative nanophage platform that merges synthetic biology with nanomedicine, offering a promising strategy against MDR bacterial infections.",
  "abstract_shown": true,
  "affiliations": [
    "Cellular Signaling Laboratory, International Research Center for Sensory Biology and Technology of MOST, Key Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China.",
    "School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Hongshan District, Wuhan 430074, Hubei, China.",
    "Department of Clinical Microbiology and Immunology, School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel.",
    "DWI-Leibniz Institute for Interactive Materials e.V., 52074 Aachen, Germany.",
    "Functional and Interactive Polymers, Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, 52074 Aachen, Germany.",
    "Aachen Maastricht Institute for Biobased Materials (AMIBM), Maastricht University, Brightlands Chemelot Campus, 6167 RD Geleen, Netherlands."
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Zhijun Chen",
      "orcid": "0009-0003-2833-8935"
    },
    {
      "affs": [
        0
      ],
      "name": "Wenjun Hu",
      "orcid": "0000-0001-8563-8844"
    },
    {
      "affs": [
        1
      ],
      "name": "Hui Nie",
      "orcid": "0000-0001-7422-4910"
    },
    {
      "affs": [
        0
      ],
      "name": "Yifan Zhang",
      "orcid": "0000-0002-2699-7369"
    },
    {
      "affs": [
        0
      ],
      "name": "Weilin Tang"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhihe Yu"
    },
    {
      "affs": [
        0
      ],
      "name": "Jiajun Cao"
    },
    {
      "affs": [
        0
      ],
      "name": "Xunying Zhu"
    },
    {
      "affs": [
        0
      ],
      "name": "Xinrong Liu",
      "orcid": "0009-0001-9597-3600"
    },
    {
      "affs": [
        0
      ],
      "name": "Mingqian Chen",
      "orcid": "0009-0000-7273-938X"
    },
    {
      "affs": [
        2
      ],
      "name": "Udi Qimron",
      "orcid": "0000-0003-2169-5270"
    },
    {
      "affs": [
        3,
        4,
        5
      ],
      "name": "Andrij Pich"
    },
    {
      "affs": [
        0
      ],
      "name": "Huan Peng",
      "orcid": "0000-0001-6799-946X"
    },
    {
      "affs": [
        0
      ],
      "name": "Jianfeng Liu",
      "orcid": "0000-0002-0284-8377"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/b57bm8dx7ss39.bib",
  "cited_by": 0,
  "doi": "10.1126/sciadv.aeh7105",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13721973/?tool=EBI",
  "id": "b57bm8dx7ss39",
  "issns": [
    "2375-2548"
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  "landing_url": "https://www.science.org/doi/abs/10.1126/sciadv.aeh7105",
  "language": "eng",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
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  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Biomedicine and Life Sciences"
    ],
    [
      "heading",
      "Health and Medicine"
    ],
    [
      "heading",
      "Materials Science"
    ],
    [
      "heading",
      "Research Article"
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      "heading",
      "SciAdv r-articles"
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  "openalex": null,
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13721973/pdf/?tool=EBI",
  "pmcid": "PMC13721973",
  "pmid": "42962024",
  "published": "2026-10-30",
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "ris": "https://research.draconitic.com/paper/b57bm8dx7ss39.ris",
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  "subjects": [
    {
      "field": "chemistry",
      "origin": "heading:Materials Science",
      "subfield": "materials-polymers"
    }
  ],
  "title": "A dual-targeting lipid nanoparticle delivery system for precision phage therapy against multidrug-resistant Gram-negative bacteria",
  "title_text": "A dual-targeting lipid nanoparticle delivery system for precision phage therapy against multidrug-resistant Gram-negative bacteria",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T12:22:34Z",
  "url": "https://research.draconitic.com/paper/b57bm8dx7ss39",
  "venue": "Science Advances",
  "versions": []
}