{
  "abstract": "His Domain Protein Tyrosine Phosphatase (HD-PTP) is required for multivesicular body (MVB) formation and endosome maturation, with Rab5-GTP levels raised upon HD-PTP depletion (Parkinson et al., 2021. doi: 10/1242/jcs.259192). Mass Spectrometry-based interactomics revealed that HD-PTP depletion increased association of Rabaptin5, a component of the early endosome Rabex5-Rabaptin5 Rab5-GEF, with multiple components of STRIPAK (striatin-interacting phosphatase and kinase). Native IPs and co-expression studies confirmed association between STRIPAK and Rabex5-Rabaptin5, including Strip1-Rabex5 and SLMAP-Rabaptin5 interactions. Consistent with functional Rabaptin5-STRIPAK links, co-depletion of Strip1 or Rabaptin5 with HD-PTP restored normal early endosome positioning and Rab5 distribution. Strip1 depletion impaired formation of central, Rab5-positive early endosomes, reduced cellular Rab5-GTP levels, and prevented the accumulation of internalised EGF-EGFR into centrally located endosomes. SLMAP depletion accentuated the central clustering of early endosomes and other endosomal compartments while also retarding the generation of new, EGFR-containing endosomes and EGFR lysosomal transport. We conclude that STRIPAK is a novel co-assembly factor of Rabex5-Rabaptin5, exerting subunit-specific effects on early endosomes.",
  "abstract_shown": true,
  "affiliations": [
    "University of Manchester"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "P. Woodman"
    },
    {
      "name": "C. Francavilla"
    },
    {
      "name": "S. N. Sugar"
    },
    {
      "name": "L. Wunderley"
    },
    {
      "name": "E. W. Trotter"
    },
    {
      "name": "H. Shimizu"
    },
    {
      "name": "G. Parkinson"
    },
    {
      "name": "P. Fullwood"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/b5b5w1vdspyy2.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.28.762573",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.28.762573v1.full",
  "id": "b5b5w1vdspyy2",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.28.762573v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "cell biology"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.28.762573v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-28",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/b5b5w1vdspyy2.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:30:37Z",
      "native_id": "10.64898/2026.10.28.762573",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.28.762573v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:cell biology",
      "subfield": "cell-biology"
    }
  ],
  "title": "STRIPAK associates with Rabex5-Rabaptin5 to promote Rab5-dependent early endosome formation and EGFR transport",
  "title_text": "STRIPAK associates with Rabex5-Rabaptin5 to promote Rab5-dependent early endosome formation and EGFR transport",
  "type": "preprint",
  "updated_at": "2026-11-01T00:20:45Z",
  "url": "https://research.draconitic.com/paper/b5b5w1vdspyy2",
  "venue": "bioRxiv",
  "versions": []
}