{
  "abstract": "The role of UBE2N in myeloid cell-mediated immune suppression in cancer remains undefined. Here, we examined the function of UBE2N in myeloid cell-mediated tumor progression using a temporally inducible myeloid-specific knockout model (LysMCreERUbe2nfl/fl ). Temporally induced deletion of Ube2n in myeloid cells (Ube2nMyeKO ) significantly hindered growth of YUMM1.7 melanoma. This was accompanied by reduced myeloid cell burden within the tumor microenvironment. We observed altered abundance of PD-1, PD-L1, and SPP1 in the Ube2nMyeKO tumor microenvironment at the tissue level. In vitro analysis showed that knock-in expression of a catalytically deficient UBE2NC87S mutant in bone marrow-derived macrophages (BMDMs) markedly decreased expression of Spp1. We observed decreased SPP1 secretion in Ube2nMyeKO BMDM-conditioned media (CM). Treatment with Ube2nMyeKO BMDM-CM decreased co-expression of PD-1, TIM-3, and LAG-3 on chronically stimulated T cells. Antibody-mediated neutralization of SPP1 in Ube2nMyeWT BMDM-CM decreased PD-1 expression on CD8+ T cells. Together, these findings suggest a role for myeloid UBE2N in YUMM1.7 progression.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Dermatology, Duke University Medical Center, Durham, NC, United States.",
    "Duke Molecular Physiology Institute, Durham, NC, United States.",
    "Division of Experimental Hematology and Cancer Biology, Cincinnati Children's Hospital, Cincinnati, OH, United States.",
    "Department of Pathology, Duke University Medical Center, Durham, NC, United States."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Kelsie Schiavone"
    },
    {
      "affs": [
        0
      ],
      "name": "Adam Pecoraro"
    },
    {
      "affs": [
        0
      ],
      "name": "Afsa Khawar"
    },
    {
      "affs": [
        0
      ],
      "name": "Kathleen Zhang"
    },
    {
      "affs": [
        1
      ],
      "name": "Vaibhav Jain"
    },
    {
      "affs": [
        1
      ],
      "name": "Simon Gregory"
    },
    {
      "affs": [
        2
      ],
      "name": "Daniel T Starczynowski"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Jennifer Y Zhang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/b60hat9vv0n8j.bib",
  "cited_by": 0,
  "doi": "10.3389/fonc.2026.1932270",
  "fulltext_url": "https://europepmc.org/articles/PMC13645565",
  "id": "b60hat9vv0n8j",
  "issns": [
    "2234-943X"
  ],
  "landing_url": "https://europepmc.org/article/MED/42851405",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2403|Immune cells in cancer"
    ]
  ],
  "openalex": "W7214181093",
  "pdf_url": "https://europepmc.org/articles/PMC13645565?pdf=render",
  "pmcid": "PMC13645565",
  "pmid": "42851405",
  "published": "2026-09-24",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/b60hat9vv0n8j.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:10Z",
      "native_id": "MED:42851405",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42851405"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2403|Immune cells in cancer",
      "subfield": "immunology"
    }
  ],
  "title": "Conditional myeloid-specific deletion of Ube2n hinders YUMM1.7 melanoma growth.",
  "title_text": "Conditional myeloid-specific deletion of Ube2n hinders YUMM1.7 melanoma growth.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:10Z",
  "url": "https://research.draconitic.com/paper/b60hat9vv0n8j",
  "venue": "Frontiers in oncology",
  "versions": []
}