{
  "abstract": "Skeletal muscle integrity and function depend on fiber-type composition and regenerative capacity. Evaluating both components in normal and injured tissues provides valuable insights into subtle declines in muscle function and recovery. We describe a protocol for the transcriptional analysis of the major fast-twitch type II myofibers and regeneration-related genes in normal and local-injured mouse Tibialis Anterior skeletal muscles. We describe steps for integrating freeze-induced injury, sample collection, tissue dissociation, RNA extraction, and gene expression analysis by quantitative reverse-transcription PCR (RT-qPCR).",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "name": "Zuleyha Yildirim"
    },
    {
      "name": "Aurélia Noll"
    },
    {
      "name": "Jean-Christophe Amé"
    },
    {
      "name": "Françoise Dantzer"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/b64d1ctgy0qd9.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104734",
  "fulltext_url": null,
  "id": "b64d1ctgy0qd9",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://univoak.hal.science/hal-05750501v1",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "1.sdv.ba"
    ],
    [
      "openalex",
      "1312|Muscle Physiology and Disorders"
    ]
  ],
  "openalex": "W7171676374",
  "pdf_url": "https://univoak.hal.science/hal-05750501/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-07-29",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/b64d1ctgy0qd9.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:34Z",
      "native_id": "hal-05750501",
      "source": "hal",
      "url": "https://univoak.hal.science/hal-05750501v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "hal:1.sdv.ba",
      "subfield": "zoology-behaviour"
    }
  ],
  "title": "Protocol for transcriptional profiling of fiber type and regeneration markers in mouse Tibialis Anterior muscle",
  "title_text": "Protocol for transcriptional profiling of fiber type and regeneration markers in mouse Tibialis Anterior muscle",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:34Z",
  "url": "https://research.draconitic.com/paper/b64d1ctgy0qd9",
  "venue": "STAR Protocols",
  "versions": []
}