{
  "abstract": "Macrophages are central regulators of innate immunity, yet the post-translational mechanisms that enable rapid inflammatory responses remain incompletely understood. Here, we integrated transcriptomics, unenriched proteomics, phosphoproteomics, metabolomics, and cysteine reactivity profiling to comprehensively characterize primary human macrophages following Toll-like receptor and Stimulator of Interferon Genes (STING) activation. This approach revealed substantial discordance between transcript- and protein-level changes, as well as extensive post-translational remodeling. Differentially reactive cysteines were enriched in proteins involved in small-GTPase signaling, vesicular trafficking, and the endo-lysosomal system. Reactivity changes in two subunits of the endosomal mRNA-transport FERRY complex nominated it as a candidate target of inflammation-associated post-translational regulation. Biochemical and imaging analyses established FERRY complex assembly and early endosomal localization in primary human macrophages and identified DISC1 as a previously unrecognized FERRY-associated protein. This work provides a comprehensive resource of biochemical and post-translational remodeling during macrophage activation and implicates endosome-associated mRNA transport in innate immune regulation.",
  "abstract_shown": true,
  "affiliations": [
    "The Rockefeller University"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "name": "T. L. Zhang"
    },
    {
      "name": "H. Sanford"
    },
    {
      "name": "R. R. Gabbasova"
    },
    {
      "name": "A. E. van Vlimmeren"
    },
    {
      "name": "Y. Kalaidzidis"
    },
    {
      "name": "H. Kojima"
    },
    {
      "name": "C. R. Wayne"
    },
    {
      "name": "J. Biswas"
    },
    {
      "name": "P. Banerjee"
    },
    {
      "name": "B. Zhang"
    },
    {
      "name": "O. Abdel-Wahab"
    },
    {
      "name": "M. Zerial"
    },
    {
      "affs": [
        0
      ],
      "name": "E. V. Vinogradova"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/b7b001ag5q8gg.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.25.754469",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.25.754469v1.full",
  "id": "b7b001ag5q8gg",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.25.754469v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "immunology"
    ],
    [
      "openalex",
      "2403|Immune Response and Inflammation"
    ]
  ],
  "openalex": "W7215034862",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.25.754469v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-25",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/b7b001ag5q8gg.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:25:40Z",
      "native_id": "10.64898/2026.09.25.754469",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.25.754469v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:immunology",
      "subfield": "immunology"
    }
  ],
  "title": "Chemical proteomics reveals post-translational remodeling of endosomal pathways during macrophage activation.",
  "title_text": "Chemical proteomics reveals post-translational remodeling of endosomal pathways during macrophage activation.",
  "type": "preprint",
  "updated_at": "2026-10-30T12:25:40Z",
  "url": "https://research.draconitic.com/paper/b7b001ag5q8gg",
  "venue": "bioRxiv",
  "versions": []
}