{
  "abstract": "Background: Periodontal regeneration requires the coordinated reconstruction of mineralized and non-mineralized tissues. P35 polypeptide derived from Cementum protein-1(CEMP1) has been demonstrated to induce bio-mineralization in vitro, although in vivo applications still need to be further validated. In this study, we developed a novel mineralizing hydrogel system (P35/ACP@PEG Hy) to facilitate periodontal regeneration by encapsulating P35-loaded amorphous calcium phosphate nanoparticles (P35/ACP-NPs) within a four-arm polyethylene glycol (PEG) hydrogel. Methods: In vitro assays were performed to characterize the physicochemical properties, sustained-release behavior, osteogenic/cementogenic effects of P35/ACP-NPs on hPDLCs, together with the mechanical performance and biocompatibility of the PEG hydrogel. The regenerative capacity of the hydrogel was further assessed in a rat periodontal defect model using micro-CT and histological staining. The regenerative efficacy was evaluated in a rat periodontal defect model via micro-CT and histological staining, while transcriptome sequencing (RNA-seq) and immune-blotting were further performed to confirm the molecular mechanisms. Results: P35/ACP@PEG exhibited favorable physicochemical properties, featuring an initial burst release followed by a sustained gradual release profile, along with the excellent biocompatibility. In vitro, P35/ACP-NPs markedly promoted osteogenic and cementogenic differentiation of hPDLCs. Correspondingly, the P35/ACP@PEG hydrogel significantly accelerated periodontal tissue regeneration in the rat model with periodontal defect. And RNA-seq analysis and immune-blotting further revealed that these effects are highly linked to activation of the TGF-β/Smad2/3/LOX signaling pathway. Conclusions: P35/ACP@PEG Hy platform can promote periodontal regeneration via activation of TGF-β/Smad2/3/LOX signaling pathway in vivo. Its sustained bioactivity, favorable biocompatibility and robust regenerative efficacy highlight a potential of P35 polypeptide for periodontal tissue repair.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Pediatric Dentistry, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing 210008, China",
    "Department of Oral Diagnostic and Surgical Sciences, Faculty of Dentistry, University of Otago, Dunedin 9016, New Zealand",
    "Department of Oral Sciences, Faculty of Dentistry, University of Otago, Dunedin 9016, New Zealand",
    "Department of Periodontology, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing 210008, China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yu Wang"
    },
    {
      "affs": [
        1
      ],
      "name": "Guangzhao Guan",
      "orcid": "0000-0001-7265-9865"
    },
    {
      "affs": [
        2
      ],
      "name": "Li Mei",
      "orcid": "0000-0001-7856-8091"
    },
    {
      "affs": [
        0
      ],
      "name": "Huiwen Zheng"
    },
    {
      "affs": [
        0
      ],
      "name": "Xianghui Xing"
    },
    {
      "affs": [
        3
      ],
      "name": "Weibin Sun"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/b7kqh5cbj1mvd.bib",
  "cited_by": 0,
  "doi": "10.3390/biom16101444",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13702467/?tool=EBI",
  "id": "b7kqh5cbj1mvd",
  "issns": [
    "2218-273X"
  ],
  "landing_url": "https://www.mdpi.com/2218-273X/16/10/1444",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2748|Periodontal Regeneration and Treatments"
    ]
  ],
  "openalex": "W7220473431",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13702467/pdf/?tool=EBI",
  "pmcid": "PMC13702467",
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/b7kqh5cbj1mvd.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:59Z",
      "native_id": "10.3390/biom16101444",
      "source": "crossref",
      "url": "https://www.mdpi.com/2218-273X/16/10/1444"
    },
    {
      "first_seen": "2026-10-30T12:21:34Z",
      "native_id": "PMC:PMC13702467",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13702467"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2748|Periodontal Regeneration and Treatments",
      "subfield": "general"
    }
  ],
  "title": "Periodontal Regeneration Using a Mineralizing Hydrogel Loaded with a Synthetic CEMP1-Derived Peptide",
  "title_text": "Periodontal Regeneration Using a Mineralizing Hydrogel Loaded with a Synthetic CEMP1-Derived Peptide",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:34Z",
  "url": "https://research.draconitic.com/paper/b7kqh5cbj1mvd",
  "venue": "Biomolecules",
  "versions": []
}