{
  "abstract": "Pancreatic ductal adenocarcinoma (PDAC) cell lines resist transfection, limiting gene-editing experiments. Here, we present a protocol for delivering CRISPR-Cas9 or prime editors into PDAC cell lines using retrovirus-derived engineered virus-like particles (eVLPs). We describe steps for generating eVLPs, packaging CRISPR-Cas9 ribonucleoproteins or prime editors with engineered prime-editing guide RNAs (epegRNAs), and transducing human PDAC cells. We use the HEK3 locus and NQO1 (NAD(P)H quinone dehydrogenase 1) exon 6 as example edits and controls, respectively. This protocol adapts readily to other genomic sites.",
  "abstract_shown": true,
  "affiliations": [
    "Liverpool Experimental Cancer Medicine Centre, University of Liverpool, Liverpool L69 3GE, UK.",
    "Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, Complutense University of Madrid, Madrid, Spain; San Carlos Health Research Institute (IdISSC), 28040 Madrid, Spain.",
    "Department of Medical Oncology, Cancer Center Amsterdam, Amsterdam University Medical Center, Vrije Universiteit, Amsterdam, the Netherlands; Cancer Pharmacology Lab, Fondazione Pisana per La Scienza, Pisa, San Giuliano Terme, Italy.",
    "School of Medicine, Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, UK.",
    "Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Complutense University of Madrid, Madrid, Spain; San Carlos Health Research Institute (IdISSC), 28040 Madrid, Spain.",
    "Liverpool Experimental Cancer Medicine Centre, University of Liverpool, Liverpool L69 3GE, UK. Electronic address: ecostell@liverpool.ac.uk.",
    "Liverpool Experimental Cancer Medicine Centre, University of Liverpool, Liverpool L69 3GE, UK. Electronic address: maglenn@liverpool.ac.uk."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Chloe Timmins-Young"
    },
    {
      "affs": [
        1
      ],
      "name": "Marco Cordani",
      "orcid": "0000-0001-9342-4862"
    },
    {
      "affs": [
        2
      ],
      "name": "Elisa Giovannetti",
      "orcid": "0000-0002-7565-7504"
    },
    {
      "affs": [
        3
      ],
      "name": "Despoina Aslanoglou"
    },
    {
      "affs": [
        4
      ],
      "name": "Alvaro Gutierrez"
    },
    {
      "affs": [
        5
      ],
      "name": "Eithne Costello"
    },
    {
      "affs": [
        6
      ],
      "name": "Mark Glenn"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/b81p90pv5t5wg.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104904",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13697262/",
  "id": "b81p90pv5t5wg",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://doi.org/10.1016/j.xpro.2026.104904",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Protocol"
    ],
    [
      "openalex",
      "1312|CRISPR and Genetic Engineering"
    ]
  ],
  "openalex": "W7222807588",
  "pdf_url": "https://europepmc.org/articles/PMC13697262?pdf=render",
  "pmcid": "PMC13697262",
  "pmid": "42891947",
  "published": "2026-10-17",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/b81p90pv5t5wg.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:21Z",
      "native_id": "PMC13697262",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.xpro.2026.104904"
    },
    {
      "first_seen": "2026-10-30T12:18:46Z",
      "native_id": "MED:42891947",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42891947"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|CRISPR and Genetic Engineering",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Protocol for eVLP-mediated CRISPR-Cas9 and prime editing in pancreatic cancer cell lines",
  "title_text": "Protocol for eVLP-mediated CRISPR-Cas9 and prime editing in pancreatic cancer cell lines",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:34:20Z",
  "url": "https://research.draconitic.com/paper/b81p90pv5t5wg",
  "venue": "STAR Protocols",
  "versions": []
}