{
  "abstract": "Temporal changes in wastewater microbiomes can reflect shifts in microbial abundance, detectable membership, and genome recovery. We paired genome-resolved and read-based metagenomics to distinguish these signals in 25 spatially pooled university wastewater samples collected during wet and dry periods. The workflow recovered 203 wet-period and 230 dry-period metagenome-assembled genomes (MAGs). Community composition differed between periods in both MAG-derived presence/absence profiles (Jaccard PERMANOVA, R2 = 0.548, p = 0.001) and read-based relative-abundance profiles (BrayCurtis PERMANOVA, R2 = 0.167, p = 0.001). Greater dry-period read-based richness persisted after rarefaction but disappeared under more stringent abundance thresholds, indicating a substantial contribution from low-abundance detections. Of 57 period-associated core species in the MAG-derived profiles, 14 had exact species-level matches in the read-based dataset. All 14 remained detectable at >=90% prevalence in the opposite period, and 13 had higher relative abundance in the period corresponding to their MAG-based association. Continued species detection therefore accompanied changes in community composition and MAG-based representation. Perfect/Strict CARD/RGI resistance-associated annotations occurred in 94.1% of wet-period and 92.2% of dry-period MAGs and were distributed across shared-core and period-associated taxa. These findings reveal repeated detection of a matched subset of microbial taxa beneath temporal community restructuring, together with widespread resistance-associated genetic potential among recovered genomes. Combining read-based and genome-resolved profiles helps distinguish changes in detectable membership from changes in the genomic characterization available for wastewater surveillance.",
  "abstract_shown": true,
  "affiliations": [
    "San Francisco State University"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "name": "G. Franco"
    },
    {
      "affs": [
        0
      ],
      "name": "A. Anand"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/b85r8pxerby9a.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.13.758942",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.13.758942v1.full",
  "id": "b85r8pxerby9a",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.13.758942v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "microbiology"
    ],
    [
      "openalex",
      "2303|Microbial Community Ecology and Physiology"
    ]
  ],
  "openalex": "W7221796893",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.13.758942v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-13",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/b85r8pxerby9a.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:27:52Z",
      "native_id": "10.64898/2026.10.13.758942",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.13.758942v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:microbiology",
      "subfield": "microbiology"
    }
  ],
  "title": "Microbial persistence beneath temporal restructuring in university wastewater metagenomes",
  "title_text": "Microbial persistence beneath temporal restructuring in university wastewater metagenomes",
  "type": "preprint",
  "updated_at": "2026-10-30T12:27:52Z",
  "url": "https://research.draconitic.com/paper/b85r8pxerby9a",
  "venue": "bioRxiv",
  "versions": []
}