{
  "abstract": "During infection, bacterial populations need to overcome low levels of host-generated H ₂ O ₂ . However, experiments almost always use high H ₂ O ₂ levels that are rarely found in hosts. Here, we use microfluidics to investigate how host-relevant H ₂ O ₂ impacts populations of the human pathogen Pseudomonas aeruginosa PA14 in flow. Using long-channel microfluidic devices, we establish that cells at the front of a population remove H ₂ O ₂ and protect their downstream neighbors. Population-level protection is mediated by three OxyR-regulated scavenging systems (KatA, KatB, AhpCF), which are each sufficient to detoxify host-relevant H ₂ O ₂ . Mutants lacking all three systems are sensitive to H ₂ O ₂ , but can be cross-protected by resistant cells when co-cultured. Cross-protection is abolished in higher flow regimes, where H ₂ O ₂ is delivered faster than cells can remove it. Our results demonstrate how local detoxification provides global protection, which results in spatial gradients across bacterial populations in flow. Together, our findings highlight how biological, chemical, and physical factors collectively determine the fate of bacterial populations in host-relevant environments.\n\nIMPORTANCE: Bacteria experience chemical and physical stresses during infection. For example, bacterial pathogens encounter low levels of host-generated H ₂ O ₂ in the bloodstream, urinary tract, and lungs. However, almost all laboratory experiments use much higher H ₂ O ₂ levels, which are uncommon in host environments. In this study, we used microfluidics to deliver host-relevant H ₂ O ₂ into populations of the human pathogen, Pseudomonas aeruginosa . We establish that P. aeruginosa uses three systems to detoxify H ₂ O ₂ , and strains lacking all three systems are highly sensitive. By co-culturing sensitive and resistant strains, we demonstrate how cells protect each other and form spatial gradients across mixed populations. Strikingly, we discover that cell–cell protection is lost in higher flow regimes, as flow-based delivery outpaces detoxification. Thus, our experiments reveal how host-relevant chemical and physical stressors combine to inhibit growth of bacterial populations.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biochemistry, University of Illinois at Urbana-Champaign"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Anuradha Sharma",
      "orcid": "0009-0003-8293-0665"
    },
    {
      "affs": [
        0
      ],
      "name": "Alexander M. Shuppara"
    },
    {
      "affs": [
        0
      ],
      "name": "Evan C. Johnson"
    },
    {
      "affs": [
        0
      ],
      "name": "Joseph E. Sanfilippo",
      "orcid": "0000-0002-8775-5179"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/b86rhf3qgtw05.bib",
  "cited_by": 0,
  "doi": "10.1128/mbio.02182-26",
  "fulltext_url": null,
  "id": "b86rhf3qgtw05",
  "issns": [
    "2150-7511"
  ],
  "landing_url": "https://journals.asm.org/doi/10.1128/mbio.02182-26",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": "https://journals.asm.org/doi/pdf/10.1128/mbio.02182-26",
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-06",
  "publisher": "American Society for Microbiology",
  "ris": "https://research.draconitic.com/paper/b86rhf3qgtw05.ris",
  "sources": [
    {
      "first_seen": "2026-11-07T00:21:17Z",
      "native_id": "10.1128/mbio.02182-26",
      "source": "crossref",
      "url": "https://journals.asm.org/doi/10.1128/mbio.02182-26"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "issn:2150-7511",
      "subfield": "microbiology"
    }
  ],
  "title": "Detoxification of host-relevant H ₂ O ₂ shapes Pseudomonas aeruginosa population gradients in flow",
  "title_text": "Detoxification of host-relevant H ₂ O ₂ shapes Pseudomonas aeruginosa population gradients in flow",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T00:22:49Z",
  "url": "https://research.draconitic.com/paper/b86rhf3qgtw05",
  "venue": "mBio",
  "versions": []
}