{
  "abstract": "Cells generate peptides absent from reference proteomes, including products of reverse proteolysis and gene fusions. These are an increasingly pursued class of targets in autoimmune disease, cancer vaccines and T-cell therapies. The sequence is itself the discovery, yet a convincing spectral match does not establish it, because an ordinary genome-encoded peptide of the same mass can explain the spectrum equally well. Independent benchmarking has identified this ambiguity as a dominant source of error in reported non-canonical identifications. Here, we introduce TransPepMS, which adjudicates each proposed assignment and returns a verdict of retained, unresolved, or rejected. For example, on adjudicating 1,154 reported proteasome-spliced immunopeptides, only 53 survived same-mass competition, whereas 91 of 99 insulin-spike hybrids were retained and all 46 planted canonical peptides were rejected, showing that TransPepMS discriminates genuine non-canonical peptides rather than merely deflating claims. TransPepMS provides peptide validation before they guide biological interpretation and immune-target selection.",
  "abstract_shown": true,
  "affiliations": [
    "University of British Columbia"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "name": "S. Y. Tabatabaei Dakhili"
    },
    {
      "name": "O. Hinse"
    },
    {
      "name": "L. Foster"
    },
    {
      "affs": [
        0
      ],
      "name": "D. Bromme"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/b8hnev0vb8ep2.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.20.760571",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.20.760571v1.full",
  "id": "b8hnev0vb8ep2",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.20.760571v1",
  "language": null,
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "biochemistry"
    ],
    [
      "openalex",
      "1607|Advanced Proteomics Techniques and Applications"
    ]
  ],
  "openalex": "W7223095983",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.20.760571v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-20",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/b8hnev0vb8ep2.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:28:57Z",
      "native_id": "10.64898/2026.10.20.760571",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.20.760571v2"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1607|Advanced Proteomics Techniques and Applications",
      "subfield": "physical"
    }
  ],
  "title": "TransPepMS: A Tool to Verify Peptides Absent from Reference Proteomes",
  "title_text": "TransPepMS: A Tool to Verify Peptides Absent from Reference Proteomes",
  "type": "preprint",
  "updated_at": "2026-10-30T12:30:05Z",
  "url": "https://research.draconitic.com/paper/b8hnev0vb8ep2",
  "venue": "bioRxiv",
  "versions": []
}