{
  "abstract": "Phosphatidylserine (PS) is a minor phospholipid constituent of high-density lipoprotein (HDL) that exhibits potent anti-inflammatory activity. It remains indeterminate whether PS incorporation can enhance anti-inflammatory effects of reconstituted HDL (rHDL). Human macrophages were treated with rHDL containing phosphatidylcholine alone (PC-rHDL) or PC and PS (PC/PS-rHDL). Interleukin (IL)-6 secretion and expression was more strongly inhibited by PC/PS-rHDL than PC-rHDL in both tumor necrosis factor (TNF)-α- and lipopolysaccharide (LPS)-stimulated macrophages. siRNA experiments revealed that the enhanced anti-inflammatory effects of PC/PS-rHDL required scavenger receptor class B type I (SR-BI). Furthermore, PC/PS-rHDL induced a greater increase in Akt1/2/3 phosphorylation than PC-rHDL. In addition, PC/PS but not PC-rHDL decreased the abundance of plasma membrane lipid rafts and p38 mitogen-activated protein kinase (p38 MAPK) phosphorylation. Finally, when these rHDL formulations were administered to dyslipidemic low-density lipoprotein (LDL)-receptor knockout mice fed a high-cholesterol diet, circulating IL-6 levels were significantly reduced only in PC/PS-rHDL-treated mice. In parallel, enhanced Akt1/2/3 phosphorylation by PC/PS-rHDL was observed in the mouse aortic tissue using immunohistochemistry. We concluded that the incorporation of PS into rHDLs enhanced their anti-inflammatory activity by modulating Akt1/2/3- and p38 MAPK-mediated signaling through SR-BI in stimulated macrophages. These data identify PS as a potent anti-inflammatory component capable of enhancing therapeutic potential of rHDL-based therapy.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 15,
  "authors": [
    {
      "name": "Maryam Darabi"
    },
    {
      "name": "Marie Lhomme"
    },
    {
      "name": "Veronica Dahik"
    },
    {
      "name": "Isabelle Guillas"
    },
    {
      "name": "Eric Frisdal"
    },
    {
      "name": "Emilie Tubeuf"
    },
    {
      "name": "Lucie Poupel"
    },
    {
      "name": "Mili Patel"
    },
    {
      "name": "Emmanuel Gautier"
    },
    {
      "name": "Thierry Huby"
    },
    {
      "name": "Maryse Guerin"
    },
    {
      "name": "Kerry-Anne Rye"
    },
    {
      "name": "Philippe Lesnik"
    },
    {
      "name": "Wilfried Le Goff"
    },
    {
      "name": "Anatol Kontush"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/bb071cgftgjjr.bib",
  "cited_by": 32,
  "doi": "10.1096/fj.201800810r",
  "fulltext_url": null,
  "id": "bb071cgftgjjr",
  "issns": [
    "0892-6638",
    "1530-6860"
  ],
  "landing_url": "https://hal.science/hal-03798360v1",
  "language": "en",
  "licence": "CC-BY-ND-4.0",
  "licence_label": "CC BY-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "2746|Cholesterol and Lipid Metabolism"
    ]
  ],
  "openalex": "W4223476319",
  "pdf_url": "https://hal.science/hal-03798360/document",
  "pmcid": null,
  "pmid": "35416331",
  "published": "2026-10-07",
  "publisher": "Federation of American Society of Experimental Biology",
  "ris": "https://research.draconitic.com/paper/bb071cgftgjjr.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:50Z",
      "native_id": "hal-03798360",
      "source": "hal",
      "url": "https://hal.science/hal-03798360v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2746|Cholesterol and Lipid Metabolism",
      "subfield": "surgery"
    }
  ],
  "title": "Phosphatidylserine enhances anti‐inflammatory effects of reconstituted HDL in macrophages via distinct intracellular pathways",
  "title_text": "Phosphatidylserine enhances anti‐inflammatory effects of reconstituted HDL in macrophages via distinct intracellular pathways",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:50Z",
  "url": "https://research.draconitic.com/paper/bb071cgftgjjr",
  "venue": "FASEB Journal",
  "versions": []
}