{
  "abstract": "The human genome is folded into chromatin loops by the cohesin complex, forming functional chromatin domains that underlie transcription and DNA replication/repair. However, how cohesin organizes these domains in living cells, especially in active euchromatin, remains elusive. To address this question, we combined single-nucleosome imaging/tracking and super-resolution 3D-structured illumination microscopy (3D-SIM) with euchromatin-specific labeling of histone H3.3. Using this nanoscopic approach, we revealed that euchromatin forms condensed domains that are constrained by cohesin-mediated loops. This organization refines the classical textbook view of euchromatin as largely open, in line with emerging evidence. Transcription machinery appears to be located near the condensed domain surfaces/borders. Cohesin loss increased nucleosome fluidity within these domains without altering their overall compaction, leading to local mixing of domains and compromising transcriptional insulation. These findings uncover an unexpected physical role of cohesin in maintaining the integrity of condensed euchromatic domains and ensuring proper higher-order regulation of gene expression.",
  "abstract_shown": true,
  "affiliations": [
    "Genome Dynamics Laboratory, National Institute of Genetics, ROIS, Mishima, Japan",
    "Graduate Institute for Advanced Studies, Graduate University for Advanced Studies (SOKENDAI), Mishima, Japan",
    "Department of Cell and Tissue Dynamics, Max Planck Institute for Molecular Biomedicine, Münster, Germany",
    "Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA",
    "Genome Evolution Laboratory, National Institute of Genetics, Mishima, Japan",
    "Division of Gene Expression Dynamics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan",
    "Fukui Institute for Fundamental Chemistry, Kyoto University, Kyoto, Japan",
    "Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA",
    "Department of Physics, Gandhi Institute of Technology and Management (GITAM) University, Bengaluru, India",
    "Division of Biological Sciences, Graduate School of Science, Kyoto University, Kyoto, Japan",
    "Department of Biological Science, Graduate School of Science, The University of Tokyo, Tokyo, Japan",
    "Molecular Cell Engineering Laboratory, National Institute of Genetics, ROIS, Mishima, Japan",
    "Laboratory for Molecular Life History, National Institute of Genetics, ROIS, Mishima, Japan",
    "Division of Transcriptomics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan",
    "Department of Biochemistry, University of Oxford, Oxford, UK",
    "Comparative Genomics Laboratory, National Institute of Genetics, ROIS, Mishima, Japan",
    "Department of Complex Systems Science, Nagoya University, Nagoya, Japan",
    "Genome Dynamics Laboratory, National Institute of Genetics, ROIS, Mishima, Japan. kmaeshim@nig.ac.jp",
    "Graduate Institute for Advanced Studies, Graduate University for Advanced Studies (SOKENDAI), Mishima, Japan. kmaeshim@nig.ac.jp"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 22,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Masa A. Shimazoe",
      "orcid": "0000-0002-2018-0497"
    },
    {
      "affs": [
        2,
        0,
        1
      ],
      "name": "Shiori Iida",
      "orcid": "0000-0002-1115-4705"
    },
    {
      "affs": [
        3,
        0,
        1
      ],
      "name": "Katsuhiko Minami",
      "orcid": "0000-0002-1750-5473"
    },
    {
      "affs": [
        4,
        1
      ],
      "name": "Koichi Higashi",
      "orcid": "0000-0002-8970-2652"
    },
    {
      "affs": [
        0
      ],
      "name": "Sachiko Tamura",
      "orcid": "0000-0002-3121-6475"
    },
    {
      "affs": [
        5
      ],
      "name": "Yoshiaki Kobayashi",
      "orcid": "0000-0002-9585-7255"
    },
    {
      "affs": [
        6
      ],
      "name": "Shin Fujishiro"
    },
    {
      "affs": [
        7
      ],
      "name": "Le Xiong"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Kako Nakazato"
    },
    {
      "affs": [
        8
      ],
      "name": "S. S. Ashwin"
    },
    {
      "affs": [
        9
      ],
      "name": "Tomoko Nishiyama",
      "orcid": "0000-0002-8349-6536"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yu Nagata",
      "orcid": "0009-0007-3130-4954"
    },
    {
      "affs": [
        10,
        1,
        11
      ],
      "name": "Masato T. Kanemaki",
      "orcid": "0000-0002-7657-1649"
    },
    {
      "affs": [
        1,
        12
      ],
      "name": "Akane Kawaguchi"
    },
    {
      "affs": [
        13
      ],
      "name": "Yasuyuki Ohkawa",
      "orcid": "0000-0001-6440-9954"
    },
    {
      "affs": [
        14
      ],
      "name": "Lothar Schermelleh",
      "orcid": "0000-0002-1612-9699"
    },
    {
      "affs": [
        15
      ],
      "name": "Atsushi Toyoda",
      "orcid": "0000-0002-0728-7548"
    },
    {
      "affs": [
        7
      ],
      "name": "Liangqi Xie",
      "orcid": "0000-0002-6546-9622"
    },
    {
      "affs": [
        4,
        1
      ],
      "name": "Ken Kurokawa",
      "orcid": "0000-0001-8662-9958"
    },
    {
      "affs": [
        5
      ],
      "name": "Hiroshi Ochiai",
      "orcid": "0000-0002-2200-1325"
    },
    {
      "affs": [
        16,
        6
      ],
      "name": "Masaki Sasai",
      "orcid": "0000-0002-1028-8242"
    },
    {
      "affs": [
        17,
        18
      ],
      "name": "Kazuhiro Maeshima",
      "orcid": "0000-0003-3909-0341"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/bbyywk272hyg3.bib",
  "cited_by": 12,
  "doi": "10.1038/s41588-026-02736-2",
  "fulltext_url": null,
  "id": "bbyywk272hyg3",
  "issns": [
    "1061-4036",
    "1546-1718"
  ],
  "landing_url": "https://doi.org/10.1038/s41588-026-02736-2",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
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      "1312|Genomics and Chromatin Dynamics"
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  "openalex": "W4413754252",
  "pdf_url": null,
  "pmcid": "PMC13001505",
  "pmid": "41867868",
  "published": "2026-09-01",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/bbyywk272hyg3.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:26:46Z",
      "native_id": "W4413754252",
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      "url": "https://doi.org/10.1038/s41588-026-02736-2"
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Genomics and Chromatin Dynamics",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Cohesin prevents local mixing of condensed euchromatic domains in living human cells",
  "title_text": "Cohesin prevents local mixing of condensed euchromatic domains in living human cells",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T13:21:07Z",
  "url": "https://research.draconitic.com/paper/bbyywk272hyg3",
  "venue": "Nature Genetics",
  "versions": []
}