{
  "abstract": "Chemical modifications of RNA bases and adenosine-to-inosine (A-to-I) editing are regulatory layers essentially unexplored in the retinal pigment epithelium (RPE), whose oxidative dysfunction underlies age-related macular degeneration. We mapped both layers in ARPE-19 cells exposed to tert-butyl hydroperoxide under two non-pharmacological interventions, Quantum Molecular Resonance (QMR) electrotherapy and a patient blood-derived secretome, across 56 libraries spanning four arms, three time points and matched stressed and stress-free conditions (GEO: GSE328981). Base modifications were called with HARMLNC and A-to-I editing screened with a transition-signal caller, then re-analysed with REDItools3, JACUSA2 and LoDEI. The high-confidence set comprised 241 calls at 15 sites, all of classes canonical to tRNA and rRNA. The most prevalent was an adenosine modification at chrM:2617 in mitochondrial 16S rRNA (MT-RNR2), detected in 51 of 56 libraries and in every arm. Two positions 1 bp apart lie in an EZR/SYTL3 overlap region, whereas a third chromosome 6 site maps to HLA-C, 127.5 Mb away. No position of either layer differed between treatment arms: 7 testable modification sites (minimum p = 0.19) and, at single-nucleotide resolution under dedicated callers agreeing to r = 0.975-0.992, 15 curated editing positions (minimum q = 0.169). Two one-sided tests bound any change in substitution composition within 1.4% and in mean editing frequency within 10% for secretome and combination and 20% for QMR. Against a stress-free baseline internal to the cohort, only chrM:13710 (MT-ND5) changed detectably (q = 0.03). These layers are therefore small, largely constitutive and quantifiably undisturbed by either intervention at the resolution available.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biomedical and Dental Sciences and Morphofunctional Imaging, Division of Medical Biotechnologies and Preventive Medicine, University of Messina, Messina, Italy.",
    "Department of Biomolecular Strategies, Genetics and Avant-Garde Therapies, Euro-Mediterranean Institute of Science and Technology (I.E.ME.S.T.), Palermo, Italy.",
    "Department of Veterinary Sciences, University of Messina, Messina, Italy.",
    "Department of Clinical and Experimental Medicine, University of Messina, Messina, Italy.",
    "Telea Electronic Engineering Srl, Sandrigo, Italy.",
    "Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Domenico Mordà"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Simona Alibrandi"
    },
    {
      "affs": [
        0
      ],
      "name": "Concetta Scimone"
    },
    {
      "affs": [
        3
      ],
      "name": "Angela D'ascola"
    },
    {
      "affs": [
        0
      ],
      "name": "Federica Aliquò"
    },
    {
      "affs": [
        0
      ],
      "name": "Giorgia Abate"
    },
    {
      "affs": [
        4
      ],
      "name": "Gianantonio Pozzato"
    },
    {
      "affs": [
        5
      ],
      "name": "Sergio Zaccaria Scalinci"
    },
    {
      "affs": [
        0
      ],
      "name": "Rosalia D'angelo"
    },
    {
      "affs": [
        0
      ],
      "name": "Antonina Sidoti"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Luigi Donato"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/bcbjdpp5gwj76.bib",
  "cited_by": 0,
  "doi": "10.1080/15476286.2026.2731889",
  "fulltext_url": "https://europepmc.org/articles/PMC13679316",
  "id": "bcbjdpp5gwj76",
  "issns": [
    "1547-6286",
    "1555-8584"
  ],
  "landing_url": "https://europepmc.org/article/MED/42876306",
  "language": "eng",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|RNA regulation and disease"
    ]
  ],
  "openalex": "W7222104523",
  "pdf_url": "https://europepmc.org/articles/PMC13679316?pdf=render",
  "pmcid": "PMC13679316",
  "pmid": "42876306",
  "published": "2026-10-15",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/bcbjdpp5gwj76.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:46Z",
      "native_id": "MED:42876306",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42876306"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|RNA regulation and disease",
      "subfield": "molecular-biology"
    }
  ],
  "title": "RNA base-modification and A-to-I editing landscapes of oxidatively stressed human retinal pigment epithelium under Quantum Molecular Resonance electrotherapy and blood-derived secretome.",
  "title_text": "RNA base-modification and A-to-I editing landscapes of oxidatively stressed human retinal pigment epithelium under Quantum Molecular Resonance electrotherapy and blood-derived secretome.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:46Z",
  "url": "https://research.draconitic.com/paper/bcbjdpp5gwj76",
  "venue": "RNA biology",
  "versions": []
}