{
  "abstract": "We have previously shown that injury to a subset of podocytes can trigger indirect damage in neighboring podocytes, but whether this phenomenon depends on direct intercellular interaction is unknown. To address this, kidney organoids were generated from nephron progenitor cells of two mouse lines: one expressing a receptor for a podocyte-specific immunotoxin and another expressing a tagged ribosomal protein. In chimeric organoids containing a mosaic of these podocyte types, immunotoxin exposure selectively injured the targeted podocytes and also induced indirect injury in adjacent, non-targeted podocytes. This was evidenced by reduced podocin staining and decreased expression of podocyte-specific genes in the non-targeted podocytes. The indirect injury was absent when organoids of each type were cultured separately but in close proximity, indicating that direct cell-to-cell contact within the same glomerular structure is required. These findings show that podocyte injury can propagate locally within kidney organoids, independent of glomerular filtration or other glomerular cell types, and suggest that local podocyte interactions may contribute to the progression of chronic kidney disease.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Physiology, Tokai University School of Medicine, Isehara, Japan",
    "Department of Pediatrics and Developmental Biology, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Japan",
    "Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
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        0
      ],
      "name": "Tomohiro Udagawa"
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        1
      ],
      "name": "Toshikazu Araoka"
    },
    {
      "affs": [
        2
      ],
      "name": "Kenji Osafune"
    },
    {
      "name": "Taiji Matsusaka"
    }
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  "bibtex": "https://research.draconitic.com/paper/bn7jgr4y6s3rm.bib",
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  "doi": "10.1371/journal.pone.0337677",
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  "id": "bn7jgr4y6s3rm",
  "issns": [
    "1932-6203"
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  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
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      "2727|Renal Diseases and Glomerulopathies"
    ],
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      "/Biology and life sciences/Anatomy/Nervous system/Synapses/Gap junctions"
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      "/Biology and life sciences/Molecular biology/Molecular biology techniques/Molecular biology assays and analysis techniques/Nucleic acid analysis/RNA analysis"
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      "/Biology and life sciences/Physiology/Electrophysiology/Neurophysiology/Synapses/Gap junctions"
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      "plos",
      "/Medicine and health sciences/Anatomy/Renal system/Kidneys"
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      "plos",
      "/Research and analysis methods/Animal studies/Experimental organism systems/Animal models/Mouse models"
    ],
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      "plos",
      "/Research and analysis methods/Animal studies/Experimental organism systems/Model organisms/Mouse models"
    ],
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      "plos",
      "/Research and analysis methods/Biological cultures/Organ cultures/Organoids"
    ],
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      "plos",
      "/Research and analysis methods/Model organisms/Mouse models"
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      "plos",
      "/Research and analysis methods/Molecular biology techniques/Molecular biology assays and analysis techniques/Nucleic acid analysis/RNA analysis"
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  "pdf_url": "https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0337677&type=printable",
  "pmcid": null,
  "pmid": null,
  "published": "2026-08-31",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/bn7jgr4y6s3rm.ris",
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    {
      "first_seen": "2026-10-30T12:18:02Z",
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      "url": "https://doi.org/10.1371/journal.pone.0337677"
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  "subjects": [
    {
      "field": "medicine",
      "origin": "plos:anatomy",
      "subfield": "physiology"
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  "title": "Podocyte injury damages podocytes in chimeric organoids",
  "title_text": "Podocyte injury damages podocytes in chimeric organoids",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:02Z",
  "url": "https://research.draconitic.com/paper/bn7jgr4y6s3rm",
  "venue": "PLOS One",
  "versions": []
}