{
  "abstract": "Background/Objectives: Anthracycline-based chemotherapy is associated with systemic toxicities in patients with breast cancer. Aerobic exercise may help mitigate treatment-related effects, but its associated metabolic responses during chemotherapy remain poorly understood. This study assessed biochemical and circulating metabolomic responses to aerobic exercise during anthracycline-based chemotherapy. Methods: This exploratory secondary analysis included 40 participants from a randomized controlled trial (trained n = 21; control, n = 19). Participants in the trained group completed a 12-week aerobic exercise intervention during chemotherapy. Biochemical markers and untargeted plasma LC–MS metabolomic profiles were assessed before and after intervention. Exploratory metabolic candidates were evaluated using linear mixed-effects models with Benjamini–Hochberg correction. Results: Significant Group × Time interactions were observed for 10 of 12 physiological and biochemical markers after BH-FDR correction in the primary analysis. However, baseline-adjusted ANCOVA sensitivity analyses showed that only IL-6 retained statistical significance after BH-FDR correction (adjusted between-group difference: −15.58 pg/mL, 95% CI: −20.72 to −10.45; FDR < 0.001). Despite limited global metabolomic separation, sequential screening identified 26 candidate features, seven of which showed significant Group × Time interactions after correction across candidates. Five had putative annotations, including lysophosphatidylcholine (LPC) 14:1, lysophosphatidylethanolamine (LPE) 17:1, cis-7-hexadecenoic acid, L-ascorbate, and vitamin E acetate. In a full-feature sensitivity analysis, only Com_3317_pos remained significant after BH-FDR correction across all 1150 metabolic features (FDR = 0.0228). Conclusions: Aerobic exercise during anthracycline-based chemotherapy was associated with changes in circulating biochemical markers, although these findings were less robust after baseline adjustment. Untargeted metabolomics identified exploratory exercise-associated metabolic features, particularly those putatively related to lipid metabolism, but most did not remain significant after full-feature multiple-testing correction. Further validation is warranted.",
  "abstract_shown": true,
  "affiliations": [
    "School of Physical Education, South-Central Minzu University, Wuhan 430074, China",
    "School of Sport Science, Beijing Sport University, Beijing 100084, China",
    "China Ice Sport College, Beijing Sport University, Beijing 100084, China",
    "Department of Breast Medicine, Sanhuan Cancer Hospital, Beijing 100021, China",
    "Key Laboratory of Physical Fitness and Exercise, Ministry of Education, Beijing Sport University, Beijing 100084, China",
    "China Institute of Sport and Health Science, Beijing Sport University, Beijing 100084, China",
    "Key Laboratory for Performance Training & Recovery of General Administration of Sport, Beijing 100084, China"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Hongmei Li"
    },
    {
      "affs": [
        1
      ],
      "name": "Jing Zuo"
    },
    {
      "affs": [
        2
      ],
      "name": "Jingzhe Xiao"
    },
    {
      "affs": [
        3
      ],
      "name": "Die Sang"
    },
    {
      "affs": [
        1,
        4
      ],
      "name": "Yimin Zhang"
    },
    {
      "affs": [
        4,
        5,
        6
      ],
      "name": "Lijing Gong",
      "orcid": "0000-0003-3502-0901"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/bnar1yz666p9y.bib",
  "cited_by": 0,
  "doi": "10.3390/metabo16110869",
  "fulltext_url": null,
  "id": "bnar1yz666p9y",
  "issns": [
    "2218-1989"
  ],
  "landing_url": "https://www.mdpi.com/2218-1989/16/11/869",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-09",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/bnar1yz666p9y.ris",
  "sources": [
    {
      "first_seen": "2026-11-10T00:21:28Z",
      "native_id": "10.3390/metabo16110869",
      "source": "crossref",
      "url": "https://www.mdpi.com/2218-1989/16/11/869"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:2218-1989",
      "subfield": "proteomics-metabolomics"
    }
  ],
  "title": "Exploratory Plasma Metabolomic Analysis of Aerobic Exercise-Associated Responses During Anthracycline-Based Chemotherapy in Patients with Breast Cancer: A Randomized Controlled Trial",
  "title_text": "Exploratory Plasma Metabolomic Analysis of Aerobic Exercise-Associated Responses During Anthracycline-Based Chemotherapy in Patients with Breast Cancer: A Randomized Controlled Trial",
  "type": "peer-reviewed",
  "updated_at": "2026-11-10T00:21:28Z",
  "url": "https://research.draconitic.com/paper/bnar1yz666p9y",
  "venue": "Metabolites",
  "versions": []
}