{
  "abstract": "Cell entry of nonenveloped animal viruses requires translocation of a macromolecular assembly across a cellular membrane. Double-stranded RNA viruses introduce into the target cell an inner capsid particle that does not uncoat further. Instead, it extrudes capped viral mRNA by virtue of polymerase and capping activities within it. As described here, we used cryogenic electron tomography to visualize the full course of rhesus rotavirus entry, from cell attachment and virion uptake to release of the subviral particle. The cryo-tomograms and subtomogram averaging of classified subparticles link high-resolution structures of the virion and its components with time series from live-cell fluorescence microscopy. We outline the mechanism of each step in the entry process, including the membrane perforation step that transfers a subviral particle into the cytosol.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School; Boston, Massachusetts, United States of America",
    "Laboratory of Molecular Medicine, Boston Children’s Hospital; Boston, Massachusetts, United States of America",
    "Harvard cryo-EM Center for Structural Biology, Harvard Medical School; Boston, Massachusetts, United States of America",
    "Department of Cell Biology, Harvard Medical School, Boston, Massachusetts; United States of America",
    "Department of Pediatrics, Harvard Medical School, Boston, Massachusetts; United States of America",
    "Program in Cellular and Molecular Medicine Boston Children’s Hospital; Boston, Massachusetts, United States of America",
    "Howard Hughes Medical Institute, Harvard Medical School; Boston, Massachusetts, United States of America"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Marilina de Sautu"
    },
    {
      "affs": [
        2
      ],
      "name": "Conny Leistner"
    },
    {
      "affs": [
        3,
        4,
        5
      ],
      "name": "Tomas Kirchhausen"
    },
    {
      "affs": [
        0
      ],
      "name": "Simon Jenni"
    },
    {
      "affs": [
        0,
        1,
        6
      ],
      "name": "Stephen C. Harrison"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/bne053mj85ana.bib",
  "cited_by": 1,
  "doi": "10.1126/science.aeg4851",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13728095/",
  "id": "bne053mj85ana",
  "issns": [
    "0036-8075",
    "1095-9203"
  ],
  "landing_url": "https://doi.org/10.1126/science.aeg4851",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "2725|Viral gastroenteritis research and epidemiology"
    ]
  ],
  "openalex": "W7212174168",
  "pdf_url": null,
  "pmcid": "PMC13728095",
  "pmid": "42721255",
  "published": "2026-09-10",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/bne053mj85ana.ris",
  "sources": [
    {
      "first_seen": "2026-11-06T12:23:50Z",
      "native_id": "PMC13728095",
      "source": "pmc",
      "url": "https://doi.org/10.1126/science.aeg4851"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2725|Viral gastroenteritis research and epidemiology",
      "subfield": "infectious-disease"
    }
  ],
  "title": "Mechanism of membrane perforation in rotavirus cell entry",
  "title_text": "Mechanism of membrane perforation in rotavirus cell entry",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T12:21:09Z",
  "url": "https://research.draconitic.com/paper/bne053mj85ana",
  "venue": "Science (New York, N.Y.)",
  "versions": []
}