{
  "abstract": "Background and Aims. Interindividual variability in response to low-dose aspirin (ASA) contributes to residual platelet activation and thrombotic risk. Platelet microRNAs (miRNAs) may modulate ASA pharmacodynamics, but mechanistic insights remain limited. To investigate whether platelet miR-144-5p regulates cyclooxygenase (COX)-1/2 expression and modulates ASA response in cardiometabolic patients. Methods. Two hundred ASA-treated patients were stratified into tertiles of serum thromboxane B₂ (sTXB₂) slope, an ex vivo marker of COX-1 recovery. Platelet and extracellular vesicle miR-144-5p levels were quantified by RT-qPCR and correlated with biochemical and clinical variables. Functional studies were performed in megakaryocytic (DAMI) and endothelial (HUVEC) cells transfected with miR-144-5p mimics or inhibitors to assess COX-1/2 regulation. Results. Patients with poor ASA response (3rd tertile of sTXB₂ slope) exhibited reduced platelet miR-144-5p (p=0.027) and higher COX-1 mRNA/protein levels (p≤0.027), whereas miR-144-5p was enriched in platelet-derived extracellular vesicles. Platelet miR-144-5p was inversely correlated with sTXB₂ (ρ=-0.235, p=0.007) and COX-1/2 mRNA expression. In DAMI (human megakaryocyte) cells, ASA exposure upregulated miR-144-5p cells; in addition, miR-144-5p overexpression suppressed, and inhibition enhanced, COX-1 protein levels. Similar effects were observed in HUVECs, where miR-144-5p downregulated both COX-1 and COX-2. Among our patients, platelet miR-144-5p was lower in those with obesity, dyslipidemia, and metabolic dysfunction-associated steatotic liver disease, conditions known to affect aspirin response. Conclusions. Platelet miR-144-5p directly regulates COX-1/2 expression and contributes to interindividual variability in ASA response. Low platelet miR-144-5p identifies patients with accelerated COX-1 recovery and residual thromboxane generation, providing a mechanistic basis for precision antiplatelet therapy and potential miRNA-targeted interventions.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 1,
  "authors": [
    {
      "name": "Paola Giustina Maria Simeone"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/bpb7c4b6trw5a.bib",
  "cited_by": 0,
  "doi": null,
  "fulltext_url": "https://www.btvb.org/btvb/article/view/779",
  "id": "bpb7c4b6trw5a",
  "issns": [
    "2785-5309"
  ],
  "landing_url": "https://www.btvb.org/btvb/article/view/779",
  "language": "en",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "RC666-701"
    ]
  ],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-26",
  "publisher": "PAGEPress Publications",
  "ris": "https://research.draconitic.com/paper/bpb7c4b6trw5a.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:47:31Z",
      "native_id": "97c12fadcb9c43beadcd10d962d5e73b",
      "source": "doaj",
      "url": "https://www.btvb.org/btvb/article/view/779"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "lcc:RC666-701",
      "subfield": "cardiovascular"
    }
  ],
  "title": "CO077 | PLATELET MIR-144-5P REGULATES COX-1/2 EXPRESSION AND MODULATES ASPIRIN RESPONSE IN PATIENTS WITH CARDIOMETABOLIC RISK",
  "title_text": "CO077 | PLATELET MIR-144-5P REGULATES COX-1/2 EXPRESSION AND MODULATES ASPIRIN RESPONSE IN PATIENTS WITH CARDIOMETABOLIC RISK",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:47:31Z",
  "url": "https://research.draconitic.com/paper/bpb7c4b6trw5a",
  "venue": "Bleeding, Thrombosis and Vascular Biology",
  "versions": []
}