{
  "abstract": "Visualizing microtubules within tissues remains technically challenging, limiting our understanding of their organization and function in vivo. Here, we present a protocol for visualizing microtubules in transzonal projections (TZPs), cellular projections that connect ovarian somatic cells and oocytes within mouse ovarian follicles. We describe steps and conditions for ovarian fixation, cryosection preparation, and immunostaining to preserve fine microtubule structures. We then detail procedures for super-resolution imaging to visualize fine microtubules within TZPs.\n\nFor complete details on the use and execution of this protocol, please refer to Aikawa et al.1",
  "abstract_shown": true,
  "affiliations": [
    "Laboratory of Cytoskeletal Logistics, Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University, Shinjuku-ku, Tokyo, Japan",
    "Institute for Medical-Oriented Structural Biology, Waseda University, Shinjuku-ku, Tokyo, Japan",
    "Laboratory for Histogenetic Dynamics, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto, Japan"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Takao Tsurumaki"
    },
    {
      "affs": [
        0
      ],
      "name": "Akihiro Aikawa"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Masamitsu Sato"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Mika Toya"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/bqntdqyacg0ww.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104924",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13735235/",
  "id": "bqntdqyacg0ww",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://doi.org/10.1016/j.xpro.2026.104924",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Protocol"
    ],
    [
      "openalex",
      "2743|Reproductive Biology and Fertility"
    ]
  ],
  "openalex": "W7224099599",
  "pdf_url": null,
  "pmcid": "PMC13735235",
  "pmid": "42949913",
  "published": "2026-10-28",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/bqntdqyacg0ww.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:20:57Z",
      "native_id": "PMC13735235",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.xpro.2026.104924"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2743|Reproductive Biology and Fertility",
      "subfield": "reproductive-child"
    }
  ],
  "title": "Protocol for visualizing microtubules in transzonal projections of mouse ovarian follicles",
  "title_text": "Protocol for visualizing microtubules in transzonal projections of mouse ovarian follicles",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:20:57Z",
  "url": "https://research.draconitic.com/paper/bqntdqyacg0ww",
  "venue": "STAR Protocols",
  "versions": []
}