{
  "abstract": "The cell membrane is the critical cell component that protects cell integrity and mediates intercellular communication. In this contribution, we develop a cell membrane-targeted bioorthogonally activatable fluorogenic probe, DCM-Tz3, by incorporating the tetrazine motif into the dicyanomethylene-4H-chromone scaffold. Upon inverse electron-demand Diels-Alder reaction, the resulting cycloadduct (DCM-Pz3) exhibits a high fluorescence turn-on ratio, near-infrared emission, and significant solvatochromic effects. Notably, DCM-Pz3 presents a two-photon excitation property with a high two-photon absorption cross-section of 113 GM. Density functional theory calculation reveals the fluorescence OFF-ON switching mechanism of the probe. Importantly, we successfully applied the combination of DCM-Tz3 and the cyclooctyne for NIR fluorescence labeling of the cell membrane in both one-photon and two-photon patterns in living cells under wash-free conditions. This work provides a valuable cell membrane imaging tool with high specificity, laying the foundation for exploring cell membrane-based biological processes.",
  "abstract_shown": true,
  "affiliations": [
    "State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, P. R. China.",
    "Theranostics and Translational Research Center, Institute of Clinical Medicine, Department of Nuclear Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Rongrong Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yingchi Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Xuemei Du"
    },
    {
      "affs": [
        0
      ],
      "name": "Hong Yang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yulin Tian",
      "orcid": "0000-0002-2355-380X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/br0w48e2dyj7r.bib",
  "cited_by": 0,
  "doi": "10.1021/cbmi.6c00053",
  "fulltext_url": "https://europepmc.org/articles/PMC13715315",
  "id": "br0w48e2dyj7r",
  "issns": [
    "2832-3637"
  ],
  "landing_url": "https://europepmc.org/article/MED/42953706",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "1607|Molecular Sensors and Ion Detection"
    ]
  ],
  "openalex": "W7163052922",
  "pdf_url": "https://europepmc.org/articles/PMC13715315?pdf=render",
  "pmcid": "PMC13715315",
  "pmid": "42953706",
  "published": "2026-06-01",
  "publisher": "American Chemical Society",
  "ris": "https://research.draconitic.com/paper/br0w48e2dyj7r.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T00:20:41Z",
      "native_id": "MED:42953706",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42953706"
    },
    {
      "first_seen": "2026-11-01T12:22:39Z",
      "native_id": "PMC13715315",
      "source": "pmc",
      "url": "https://doi.org/10.1021/cbmi.6c00053"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1607|Molecular Sensors and Ion Detection",
      "subfield": "physical"
    }
  ],
  "title": "Design of Cell Membrane-Targeted Two-Photon Excited Bioorthogonal Near-Infrared Fluorogenic Probes.",
  "title_text": "Design of Cell Membrane-Targeted Two-Photon Excited Bioorthogonal Near-Infrared Fluorogenic Probes.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T12:22:39Z",
  "url": "https://research.draconitic.com/paper/br0w48e2dyj7r",
  "venue": "Chemical & biomedical imaging",
  "versions": []
}