{
  "abstract": "Enterotoxigenic Bacteroides fragilis (ETBF) secretes Bacteroides fragilis toxin (BFT), a metalloprotease that disrupts intestinal epithelial integrity and activates pro-inflammatory signaling. Although E-cadherin cleavage is a well-established response to BFT, the involvement of other membrane receptors in BFT-induced signaling remains poorly defined. In this study, we examined whether HER2 undergoes proteolytic processing following BFT exposure and whether HER2 contributes to BFT-induced ERK activation. In HT29/c1 and MCF-7 epithelial cells, BFT induced the loss of full-length 185 kDa HER2 and the generation of a membrane-tethered ~95 kDa fragment. This truncated HER2 underwent γ-secretase-dependent intramembrane processing and subsequent proteasomal degradation. Knockdown of MMP-7 or ADAM10 did not prevent HER2 cleavage, indicating that neither protease is required for this process. In addition, BFT-induced ERK phosphorylation was observed in HER2-expressing cells but was abolished in HER2-deficient HT29/c1 clones, whereas E-cadherin cleavage and IL-8 secretion remained detectable. These findings identify HER2 as a previously unrecognized target of BFT-induced proteolytic remodeling and support a contribution of HER2 to BFT-induced ERK activation.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju 26493, Republic of Korea.",
    "Department of Obstetrics, Gynecology & Women's Health, University of Missouri School of Medicine, Columbia, MO 65211, USA.",
    "Medical Device Business Division, ECO DM LAB Co., Ltd., Cheongju 28121, Republic of Korea.",
    "Regeneration Medicine Research Center, Yonsei University Wonju College of Medicine, Wonju 26426, Republic of Korea."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Sang-Hyeon Yoo",
      "orcid": "0000-0001-5430-8383"
    },
    {
      "affs": [
        0
      ],
      "name": "Ha-Neul Jang",
      "orcid": "0009-0002-9701-5008"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Da-Hye Kang",
      "orcid": "0000-0001-8002-5037"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Ju-Eun Hong",
      "orcid": "0000-0002-5357-4476"
    },
    {
      "affs": [
        0
      ],
      "name": "Jiyun Hong",
      "orcid": "0000-0003-3278-0710"
    },
    {
      "affs": [
        3
      ],
      "name": "Young Woo Eom",
      "orcid": "0000-0002-5985-6490"
    },
    {
      "affs": [
        0
      ],
      "name": "Ki-Jong Rhee",
      "orcid": "0000-0001-5130-2728"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/brhbm5c315zq4.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27198497",
  "fulltext_url": "https://europepmc.org/articles/PMC13665071",
  "id": "brhbm5c315zq4",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://europepmc.org/article/MED/42883746",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2730|HER2/EGFR in Cancer Research"
    ]
  ],
  "openalex": "W7214125205",
  "pdf_url": "https://europepmc.org/articles/PMC13665071?pdf=render",
  "pmcid": "PMC13665071",
  "pmid": "42883746",
  "published": "2026-09-23",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/brhbm5c315zq4.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:16Z",
      "native_id": "MED:42883746",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42883746"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2730|HER2/EGFR in Cancer Research",
      "subfield": "oncology"
    }
  ],
  "title": "Bacteroides fragilis Toxin Induces Extracellular Domain Cleavage of HER2 in Intestinal Epithelial Cells.",
  "title_text": "Bacteroides fragilis Toxin Induces Extracellular Domain Cleavage of HER2 in Intestinal Epithelial Cells.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:16Z",
  "url": "https://research.draconitic.com/paper/brhbm5c315zq4",
  "venue": "International journal of molecular sciences",
  "versions": []
}