{
  "abstract": "BackgroundMirror therapy (MT)--specifically paradigms using mirror visual feedback (MVF)--is widely used in neurorehabilitation; however, mechanistic implementations vary substantially in movement content, rhythmicity and attentional demands. This protocol describes an acute mechanistic, within-participant fNIRS screening study designed to compare three prespecified upper-limb mirror-therapy task paradigms and to quantify associated subjective experience after each condition in healthy adults during a single visit.\n\nMethods and analysisThis is a single-centre, within-participant, randomised crossover study conducted at Wuhan Wuchang Hospital (Wuhan, China). Healthy adults aged 18-35 years will complete three task conditions once each in a counterbalanced order using a 3x3 Latin-square scheme: UMT1 (task-oriented rhythmic functional movement), UMT2 (open-ended free movement with auditory control), and UMT3 (non-functional rhythmic movement). fNIRS will be acquired using the NirSmart-6000A system during a standardised block design. The primary outcome is ROI-level HbO activation quantified as GLM-derived {beta} estimates within the prespecified primary ROIs (bilateral SM1/M1 and bilateral PMC). Secondary outcomes include ROI-level windowed {Delta}HbO (5-20 s post-onset relative to the immediately preceding rest; descriptive only), ROI-level {Delta}HbR, and post-condition subjective ratings (illusion, immersion, confusion and fatigue; 1-7 Likert). Condition effects will be analysed using linear mixed-effects models with fixed effects for condition and period and prespecified multiplicity-adjusted pairwise contrasts.\n\nEthics and disseminationEthics approval was obtained from the Ethics Committee of Wuchang Hospital Affiliated to Wuhan University of Science and Technology (Approval No.: 2025-112-01; approved on 2025-08-21). The study is expected to be minimal risk. Findings will be disseminated through publication of this protocol manuscript and subsequent results manuscripts and conference presentations.\n\nTrial registration numberChinese Clinical Trial Registry (ChiCTR2600116634). This study is conducted as a prespecified mechanistic sub-study under the overarching registered project.\n\nStrengths and limitations of this studyO_LIWithin-participant randomised crossover design reduces between-participant variability for mechanistic comparisons. C_LIO_LIPrespecified task timing, ROIs, contrasts and multiplicity control reduce analytic flexibility. C_LIO_LILatin-square counterbalancing and modelling of period effects mitigate systematic sequence bias. C_LIO_LIHealthy-participant, single-session design limits direct generalisability to clinical stroke populations and long-term treatment effects. C_LIO_LIfNIRS remains sensitive to motion and scalp-optode coupling; residual artefacts may persist despite prespecified QC and preprocessing. C_LI",
  "abstract_shown": true,
  "affiliations": [
    "Wuchang Hospital Affiliated to Wuhan University of Science and Technology; The First Affiliated Hospital of Chongqing Medical University"
  ],
  "article_type": "PUBLISHAHEADOFPRINT",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "T. Yang"
    },
    {
      "name": "S. Wei"
    },
    {
      "name": "Y. Wang"
    },
    {
      "name": "D. Bai"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/bsg8wt8emvcg2.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.08.28.26361691",
  "fulltext_url": "https://www.medrxiv.org/content/10.64898/2026.08.28.26361691v1.full",
  "id": "bsg8wt8emvcg2",
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  "landing_url": "https://www.medrxiv.org/content/10.64898/2026.08.28.26361691v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "medrxiv",
      "rehabilitation medicine and physical therapy"
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    [
      "openalex",
      "2742|Stroke Rehabilitation and Recovery"
    ]
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  "openalex": "W7206156436",
  "pdf_url": "https://www.medrxiv.org/content/10.64898/2026.08.28.26361691v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-08-28",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/bsg8wt8emvcg2.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:35Z",
      "native_id": "10.64898/2026.08.28.26361691",
      "source": "medrxiv",
      "url": "https://www.medrxiv.org/content/10.64898/2026.08.28.26361691v1"
    }
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  "subjects": [
    {
      "field": "medicine",
      "origin": "medrxiv:rehabilitation medicine and physical therapy",
      "subfield": "general"
    }
  ],
  "title": "A single-session randomised crossover fNIRS study comparing three upper-limb mirror therapy task paradigms in healthy adults: a study protocol",
  "title_text": "A single-session randomised crossover fNIRS study comparing three upper-limb mirror therapy task paradigms in healthy adults: a study protocol",
  "type": "preprint",
  "updated_at": "2026-10-30T12:17:35Z",
  "url": "https://research.draconitic.com/paper/bsg8wt8emvcg2",
  "venue": "medRxiv",
  "versions": []
}