{
  "abstract": "Introduction: Recent work has shown that Eubacterium limosum suppresses colorectal cancer (CRC), but the contribution and cellular actions of its short-chain fatty acids remain incompletely defined.\n\nMethods: We characterized E. limosum KGMB01548, a human gut isolate, and examined the antitumor activity of its cell-free culture supernatant (ElCFS).\n\nResults: ElCFS preferentially inhibited the proliferation of human HCT116 and murine CT26 CRC cells while having little effect on non-malignant CCD 841 CoN colonic cells. The activity persisted after heat and enzymatic treatments, and pH-dependent liquid–liquid extraction indicated that a small organic acid was a major bioactive component. High-performance liquid chromatography detected 13.77 mM butyrate and 23.33 mM acetate in ElCFS. At approximately the concentration delivered by 10% ElCFS, sodium butyrate (NaB; 1.3 mM) produced comparable antiproliferative activity, whereas sodium acetate did not, supporting butyrate as a major candidate contributor to ElCFS activity. ElCFS and approximately concentration-matched NaB increased histone H3 acetylation, consistent with histone deacetylase inhibition, and induced apoptosis, as evidenced by PARP and caspase-3 cleavage and p53 phosphorylation. Their antiproliferative effects were markedly attenuated under glucose-depleted conditions. In a CT26 syngeneic tumor model, peritumoral administration of ElCFS reduced tumor growth, indicating that secreted E. limosum metabolites retained local antitumor activity outside the intestinal route. Oral pre-administration of live E. limosum also reduced subsequent tumor burden. Furthermore, 16S rRNA gene profiling suggested modest changes in several putative short-chain-fatty-acid-producing taxa and in PICRUSt2-predicted butyrate-biosynthesis potential, without a major change in alpha diversity.\n\nDiscussion: Together, these findings link E. limosum KGMB01548 to butyrate-associated histone acetylation, apoptosis, glucose-sensitive antiproliferative activity, and tumor suppression, supporting further investigation of this gut commensal and its secreted metabolites as microbiome-derived approaches for CRC control.",
  "abstract_shown": true,
  "affiliations": [
    "Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology",
    "University of Science and Technology (UST)",
    "Department of Biomedical Science, Sunchon National University"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Seung Yeob Yu"
    },
    {
      "affs": [
        0
      ],
      "name": "Jeong Eun Bak"
    },
    {
      "affs": [
        0
      ],
      "name": "Byeong Seob Oh"
    },
    {
      "affs": [
        0
      ],
      "name": "Seoung Woo Ryu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Hyebin Park"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Dong Jin Yu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jiyoung Lee"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Ji-Sun Kim"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Ju Huck Lee"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/bsnnxnyzy8695.bib",
  "cited_by": 0,
  "doi": "10.3389/fmicb.2026.1973432",
  "fulltext_url": "https://europepmc.org/articles/PMC13680443",
  "id": "bsnnxnyzy8695",
  "issns": [
    "1664-302X"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fmicb.2026.1973432/full",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Gut microbiota and health"
    ]
  ],
  "openalex": "W7220743687",
  "pdf_url": "https://europepmc.org/articles/PMC13680443?pdf=render",
  "pmcid": "PMC13680443",
  "pmid": "42903311",
  "published": "2026-10-06",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/bsnnxnyzy8695.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:12Z",
      "native_id": "10.3389/fmicb.2026.1973432",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fmicb.2026.1973432/full"
    },
    {
      "first_seen": "2026-10-30T12:19:57Z",
      "native_id": "MED:42903311",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42903311"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Gut microbiota and health",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Butyrate-associated antiproliferative and antitumor effects of Eubacterium limosum KGMB01548 in colorectal cancer",
  "title_text": "Butyrate-associated antiproliferative and antitumor effects of Eubacterium limosum KGMB01548 in colorectal cancer",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:57Z",
  "url": "https://research.draconitic.com/paper/bsnnxnyzy8695",
  "venue": "Frontiers in Microbiology",
  "versions": []
}