{
  "abstract": "Biodosimetry is essential for absorbed-dose estimation following occupational radiation accidents where early clinical decisions determine whether individuals require hospitalization or can be managed on an outpatient basis. Radioinduced transcriptomic alterations are a promising tool for rapid dose estimation. We established a gene expression panel for dose classification at 4 h post-exposure, reducing the time typically required for cytogenetic assays. Peripheral blood leukocytes from healthy individuals were X-ray irradiated and cultured for 4 h. RNA was sequenced and reads were mapped to the human hg38 genome (STAR software). Differentially expressed genes (DEGs) were identified using DESeq2 (false discovery rate, FDR, < 0.05). Radiation specificity was confirmed using eight independent microarray datasets from multiple disease conditions. DEGs were validated by RT-qPCR. A total of 215 DEGs were identified; hierarchical clustering separated samples by absorbed dose. AEN, CD70, CDKN1A, DDB2, FDXR, PCNA, and VPREB3 showed strong dose-dependent transcriptional changes across multiple disease conditions, demonstrating specificity to IR. A LASSO logistic regression model discriminated between low-dose (0–100 cGy) and high-dose (≥ 200 cGy) exposures, achieving AUC-ROC = 0.88 with CDKN1A and CD70, and AUC-ROC = 1.00 with CDKN1A, CD70, and DDB2. This gene panel offers a reliable tool for early classification of radiation exposure, supporting improved treatment of exposed individuals.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "name": "Luisa V. Biolatti"
    },
    {
      "name": "Maria S. Ausas"
    },
    {
      "name": "Jerónimo Leberle"
    },
    {
      "name": "Damián G. Hernández"
    },
    {
      "name": "Lara Negrin"
    },
    {
      "name": "Laura Mazzitelli-Fuentes"
    },
    {
      "name": "Adriana Cascon"
    },
    {
      "name": "Ananya Choudhury"
    },
    {
      "name": "Nicolas Bellora"
    },
    {
      "name": "Irene L. Ibañez"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/bt5ynt1w4jcqr.bib",
  "cited_by": 0,
  "doi": "10.1038/s41598-026-75083-1",
  "fulltext_url": null,
  "id": "bt5ynt1w4jcqr",
  "issns": [
    "2045-2322"
  ],
  "landing_url": "https://www.nature.com/articles/s41598-026-75083-1",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2741|Effects of Radiation Exposure"
    ]
  ],
  "openalex": "W7223245595",
  "pdf_url": "https://www.nature.com/articles/s41598-026-75083-1_reference.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-22",
  "publisher": "Springer Science and Business Media LLC",
  "ris": "https://research.draconitic.com/paper/bt5ynt1w4jcqr.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:33Z",
      "native_id": "10.1038/s41598-026-75083-1",
      "source": "crossref",
      "url": "https://www.nature.com/articles/s41598-026-75083-1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2741|Effects of Radiation Exposure",
      "subfield": "imaging"
    }
  ],
  "title": "Early dose stratification 4 h after ionizing radiation exposure using a peripheral blood transcriptional gene panel",
  "title_text": "Early dose stratification 4 h after ionizing radiation exposure using a peripheral blood transcriptional gene panel",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:33Z",
  "url": "https://research.draconitic.com/paper/bt5ynt1w4jcqr",
  "venue": "Scientific Reports",
  "versions": []
}