{
  "abstract": "Rhinogobio nasutus, a freshwater fish belonging to the family Cyprinidae, is primarily distributed in the upper and middle reaches of the Yellow River. However, overfishing and habitat degradation have resulted in a marked decline in its wild populations, and the species was listed as a Class II nationally protected aquatic wildlife species in China in 2021. Spermatogonial stem cells (SSCs) provide a valuable resource for fish germplasm conservation and reproductive biotechnology. In this study, we successfully established a long-term cultured germ-cell population with spermatogonial stem cell-like characteristics derived from R. nasutus (RnSSCs). These cells exhibited a morphology consistent with an undifferentiated spermatogonial phenotype and showed a diploid chromosome number of 2n = 50. Alkaline phosphatase (AP) staining showed strong positive activity, while RT-PCR detected the expression of germ-cell- and stem-cell-associated markers, with only weak expression of the somatic-cell-associated marker. Immunofluorescence staining revealed strong signals for both proliferating cell nuclear antigen (PCNA) and the germ cell-specific protein Vasa. After more than four months of in vitro culture, the cells continued to proliferate and retained expression of germ-cell- and stem-cell-associated markers. Following transplantation into zebrafish blastulae, fluorescently labelled cells were detected in the gonadal region of a small number of recipient embryos. Following hormonal induction, some cells developed a sperm-like morphology, accompanied by the expression of the meiotic-associated markers rec8 and dmc1. The establishment of the RnSSC line provides a renewable cellular resource for studying spermatogenesis and germ cell transplantation in R. nasutus. These findings provide a useful cellular resource for studying the reproductive biology of R. nasutus.",
  "abstract_shown": true,
  "affiliations": [
    "Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, College of Fisheries and Life Science, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China.",
    "Ningxia Fisheries Research Institute, Yinchuan 750001, China.",
    "Ningxia Fishery Engineering Technology Research Center, Yinchuan 750001, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yue Wang"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Yanbin Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Muhammad Jawad"
    },
    {
      "affs": [
        0
      ],
      "name": "Haijing Xu",
      "orcid": "0000-0001-8495-946X"
    },
    {
      "affs": [
        0
      ],
      "name": "Qinjun Wu"
    },
    {
      "affs": [
        0
      ],
      "name": "Lang Gui"
    },
    {
      "affs": [
        0
      ],
      "name": "Ni Hong",
      "orcid": "0000-0002-9404-3110"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Zongqiang Lian"
    },
    {
      "affs": [
        0
      ],
      "name": "Mingyou Li",
      "orcid": "0000-0002-8729-7964"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/btb8qrm5bmj1r.bib",
  "cited_by": 0,
  "doi": "10.3390/biology15191779",
  "fulltext_url": "https://europepmc.org/articles/PMC13664395",
  "id": "btb8qrm5bmj1r",
  "issns": [
    "2079-7737"
  ],
  "landing_url": "https://europepmc.org/article/MED/42883452",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1314|Reproductive biology and impacts on aquatic species"
    ]
  ],
  "openalex": "W7220912812",
  "pdf_url": "https://europepmc.org/articles/PMC13664395?pdf=render",
  "pmcid": "PMC13664395",
  "pmid": "42883452",
  "published": "2026-10-07",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/btb8qrm5bmj1r.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:00Z",
      "native_id": "MED:42883452",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42883452"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1314|Reproductive biology and impacts on aquatic species",
      "subfield": "physiology"
    }
  ],
  "title": "Establishment and Characterization of a Spermatogonial Stem Cell-like Line with Potential for Meiotic Differentiation in the Endangered Fish Rhinogobio nasutus.",
  "title_text": "Establishment and Characterization of a Spermatogonial Stem Cell-like Line with Potential for Meiotic Differentiation in the Endangered Fish Rhinogobio nasutus.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:00Z",
  "url": "https://research.draconitic.com/paper/btb8qrm5bmj1r",
  "venue": "Biology",
  "versions": []
}