{
  "abstract": "Insufficient sleep is a recognized risk factor for inflammatory diseases, yet how sleep loss functionally perturbs immune regulation remains unclear. Here, we apply a multi-omics framework combining two-sample Mendelian randomization, sleep-restriction intervention microarray, and PBMC single-cell profiling to dissect immune consequences of sleep loss. Mendelian randomization associated genetically proxied sleeplessness/insomnia symptoms with alterations in 162 plasma proteins and increased risk across 94 clinical diagnosis traits. Gene expression microarray after sleep restriction confirmed immune activation and stress-response programs. Single-cell RNA sequencing revealed PBMC remodeling with increased NK/NKT cells and decreased γδ T cells and dendritic cells, alongside pro-inflammatory pathway activity. Ligand–receptor analysis identified altered intercellular communication patterns, characterized by increasing signals to NK cells and enhancing NK–T cell-associated interfaces. These findings nominate dysregulated NK cell–T cell crosstalk as a putative mechanism underlying sleep-related inflammation and provide prioritized targets for future validation.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Genetics, Zhejiang University International School of Medicine, Hangzhou, Zhejiang China",
    "School of Computer Science and Technology, Beijing Jiaotong University, Beijing, China",
    "Center for Genetic Medicine, The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu, Zhejiang China",
    "Department of Human Genetics, and Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Zhengyang Qiu"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Jichen Jin"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Pengqi Zhang"
    },
    {
      "affs": [
        3
      ],
      "name": "Chen Li"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Yongmei Xi"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/btq35t9a9evpf.bib",
  "cited_by": 0,
  "doi": "10.1038/s41540-026-00787-z",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13737279/",
  "id": "btq35t9a9evpf",
  "issns": [
    "2056-7189"
  ],
  "landing_url": "https://doi.org/10.1038/s41540-026-00787-z",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "2805|Sleep and Wakefulness Research"
    ]
  ],
  "openalex": "W7168239448",
  "pdf_url": "https://www.nature.com/articles/s41540-026-00787-z_reference.pdf",
  "pmcid": "PMC13737279",
  "pmid": "42449104",
  "published": "2026-07-14",
  "publisher": "Nature Publishing Group",
  "ris": "https://research.draconitic.com/paper/btq35t9a9evpf.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:22:31Z",
      "native_id": "PMC13737279",
      "source": "pmc",
      "url": "https://doi.org/10.1038/s41540-026-00787-z"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2805|Sleep and Wakefulness Research",
      "subfield": "neuroscience"
    }
  ],
  "title": "Sleep restriction reprograms systemic immunity through NK and T cell crosstalk",
  "title_text": "Sleep restriction reprograms systemic immunity through NK and T cell crosstalk",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:22:31Z",
  "url": "https://research.draconitic.com/paper/btq35t9a9evpf",
  "venue": "NPJ Systems Biology and Applications",
  "versions": []
}