{
  "abstract": "An effective dengue vaccine must establish durable immunity across four dengue virus (DENV) serotypes, evade antibody-dependent enhancement (ADE), and overcome immune imprinting from prior exposure. Here, we developed TetprM-E mRNA-LNP, a tetravalent vaccine comprising LNP-formulated nucleoside-modified mRNAs encoding DENV1-4 precursor membrane-envelope (prM-E) antigens, and evaluated its immunogenicity, durability, protection and breath across serotype-specific immune backgrounds. Three immunization strategies elicited neutralizing titers greater than 1:1500 against each serotype. Passive transfer of immune sera conferred 100% survival and reduced viremia after lethal DENV2 challenge in immunocompromised mice. Vaccination induced DENV envelope (env)-specific CD8+T cells and CD4-dependent germinal-center responses. DENV env-binding antibodies persisted for 34 weeks alongside DENV env-specific memory B cells. Vaccine-elicited sera showed no measurable in vitro ADE. Although monovalent priming initially biased antibody responses, TetprM-E mRNA-LNP vaccination broadened neutralization across DENV1-4 regardless of priming serotype. Together, TetprM-E mRNA-LNP elicits broad, durable, and protective immunity despite serologic constraints from pre-existing immunity.",
  "abstract_shown": true,
  "affiliations": [
    "Yale School of Medicine"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 17,
  "authors": [
    {
      "name": "F. Akther"
    },
    {
      "name": "Y. R. Dalben"
    },
    {
      "name": "J. Villalpando"
    },
    {
      "name": "K. Levine"
    },
    {
      "name": "E. Buck"
    },
    {
      "name": "J. Polster"
    },
    {
      "name": "Z. Yin"
    },
    {
      "name": "H. Muramatsu"
    },
    {
      "name": "S. Tiwari"
    },
    {
      "name": "S. Sriram"
    },
    {
      "name": "C. B. Wilen"
    },
    {
      "name": "N. S. Joshi"
    },
    {
      "name": "R. P. McNamara"
    },
    {
      "name": "D. Weiskopf"
    },
    {
      "name": "L. V. Tse"
    },
    {
      "name": "N. Pardi"
    },
    {
      "affs": [
        0
      ],
      "name": "D. R. Martinez"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/bw7hvaj25rkdm.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.11.09.765416",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.11.09.765416v1.full",
  "id": "bw7hvaj25rkdm",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.11.09.765416v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "immunology"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.11.09.765416v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-09",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/bw7hvaj25rkdm.ris",
  "sources": [
    {
      "first_seen": "2026-11-11T12:23:58Z",
      "native_id": "10.64898/2026.11.09.765416",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.11.09.765416v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:immunology",
      "subfield": "immunology"
    }
  ],
  "title": "Tetravalent dengue mRNA vaccination orchestrates balanced four-serotype immunity with durable memory and protection in mice.",
  "title_text": "Tetravalent dengue mRNA vaccination orchestrates balanced four-serotype immunity with durable memory and protection in mice.",
  "type": "preprint",
  "updated_at": "2026-11-13T00:22:43Z",
  "url": "https://research.draconitic.com/paper/bw7hvaj25rkdm",
  "venue": "bioRxiv",
  "versions": []
}