{
  "abstract": "The generation of agricultural and food-processing byproducts has increased rapidly, highlighting the importance of their high-value utilization. In this study, a putative chitinase gene was identified from a metagenome-assembled genome. The enzyme plays a key role in chitin hydrolysis and was heterologously expressed in Escherichia coli as a recombinant protein with a molecular weight of approximately 39.2 kDa. The chitinase exhibited optimal activity toward colloidal chitin at pH 6.0 and 40 °C. Enzyme activity was significantly enhanced by Mn2+, Mg2+, and Sr2+, remained largely unaffected by Ca2+, and was strongly inhibited by Co2+ and Fe3+, while Zn2+ and Cu2+ completely abolished activity. Site-directed mutagenesis confirmed the conserved catalytic motif G-DG-D-D-E. Furthermore, the enzyme was applied to the direct hydrolysis of crayfish shell waste without conventional acid–alkali pretreatment, resulting in the formation of N-acetyl-d-glucosamine at a concentration of 3.26 mg/g. This approach eliminates the requirement for conventional acid–alkali pretreatment and enables the efficient valorization of crustacean shell waste. The developed enzymatic strategy provides a sustainable route for producing functional amino sugars with potential applications in food and related industries.",
  "abstract_shown": true,
  "affiliations": [
    "Hubei University of Technology , , ,",
    "Northwest Institute of Plateau Biology, Chinese Academy of Sciences , , ,",
    "Konkuk University , , 1 Hwayang-Dong, Gwangjin-Gu , ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Shuo Xia"
    },
    {
      "affs": [
        0
      ],
      "name": "Changhan Yu"
    },
    {
      "affs": [
        0
      ],
      "name": "Lulu Cao"
    },
    {
      "affs": [
        1
      ],
      "name": "Hongtao Bi"
    },
    {
      "affs": [
        2
      ],
      "name": "Jung-Kul Lee",
      "orcid": "0000-0001-7384-5301"
    },
    {
      "affs": [
        2
      ],
      "name": "Vipin Chandra Kalia"
    },
    {
      "affs": [
        0
      ],
      "name": "Chunjie Gong",
      "orcid": "0000-0003-2198-6342"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/bx9vt6dgv9z1b.bib",
  "cited_by": 0,
  "doi": "10.1021/acsomega.6c08438",
  "fulltext_url": "https://europepmc.org/articles/PMC13689097",
  "id": "bx9vt6dgv9z1b",
  "issns": [
    "2470-1343"
  ],
  "landing_url": "https://pubs.acs.org/acsodf/article/11/41/63866/5447006/Identification-and-Characterization-of-a",
  "language": "eng",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Studies on Chitinases and Chitosanases"
    ]
  ],
  "openalex": "W7220944544",
  "pdf_url": "https://pubs.acs.org/doi/pdf/10.1021/acsomega.6c08438",
  "pmcid": "PMC13689097",
  "pmid": "42924103",
  "published": "2026-10-08",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/bx9vt6dgv9z1b.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:39Z",
      "native_id": "10.1021/acsomega.6c08438",
      "source": "crossref",
      "url": "https://pubs.acs.org/acsodf/article/11/41/63866/5447006/Identification-and-Characterization-of-a"
    },
    {
      "first_seen": "2026-10-30T12:20:36Z",
      "native_id": "MED:42924103",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42924103"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Studies on Chitinases and Chitosanases",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Identification and Characterization of a Previously Uncharacterized Metagenome-Derived Chitinase and Its Application in N -Acetyl- d -Glucosamine Production from Crayfish Shell Waste",
  "title_text": "Identification and Characterization of a Previously Uncharacterized Metagenome-Derived Chitinase and Its Application in N -Acetyl- d -Glucosamine Production from Crayfish Shell Waste",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:36Z",
  "url": "https://research.draconitic.com/paper/bx9vt6dgv9z1b",
  "venue": "ACS Omega",
  "versions": []
}