{
  "abstract": "Automated measurements of anisokaryosis in canine cutaneous mast cell tumors (ccMCTs) have been shown to be predictive of survival, but questions remain regarding the intratumoral distribution of anisokaryosis. Whole-slide images of 96 ccMCTs were analyzed with a deep learning-based segmentation algorithm to quantify anisokaryosis using the standard deviation (SD) of the nuclear area. In 35/96 cases, >5% of the non-overlapping 256 × 256 µm2 regions were hotspots (SD ≥11.5 µm2). Regions selected by 7 pathologists within these 35 cases matched hotspots in 32% of the instances. Outcome analysis (tumor-related death) based on single tumor regions yielded an area under the curve (AUC) of 0.901 for pathologist-selected hotspots, falling between random region selection (AUC: 0.862) and 90th-percentile targeted selection (AUC: 0.956). Whole-slide analysis of the hotspot proportion predicted survival with an AUC of 0.956, with 20% of hotspots as a prognostically meaningful threshold. Whereas pathologists-selected tumor regions are prognostically meaningful for nuclear morphometry, whole-slide analysis may provide additional prognostic information.",
  "abstract_shown": true,
  "affiliations": [
    "University of Veterinary Medicine Vienna, Vienna, Austria",
    "Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany",
    "Mira Vision Microscopy GmbH, Göppingen, Germany",
    "The Schwarzman Animal Medical Center, New York, NY",
    "University of Applied Sciences Upper Austria, Hagenberg, Austria",
    "Johannes Kepler University Linz, Linz, Austria",
    "Ross University School of Veterinary Medicine, Basseterre, Saint Kitts and Nevis",
    "University of Florida, Gainesville, FL",
    "Freie Universität Berlin, Berlin, Germany",
    "Hochschule Flensburg, Flensburg, Germany",
    "Michigan State University, Lansing, MI"
  ],
  "article_type": "brief report",
  "arxiv": null,
  "author_total": 15,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Christof A. Bertram",
      "orcid": "0000-0002-2402-9997"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Frauke Wilm",
      "orcid": "0000-0002-9065-0554"
    },
    {
      "affs": [
        0
      ],
      "name": "Eda Parlak"
    },
    {
      "affs": [
        3
      ],
      "name": "Taryn A. Donovan",
      "orcid": "0000-0001-5740-9550"
    },
    {
      "affs": [
        4,
        5
      ],
      "name": "Hannah Janout"
    },
    {
      "affs": [
        6
      ],
      "name": "Pompei Bolfa",
      "orcid": "0000-0002-2903-1535"
    },
    {
      "affs": [
        7
      ],
      "name": "Michael J. Dark",
      "orcid": "0000-0002-6547-1298"
    },
    {
      "affs": [
        0
      ],
      "name": "Andrea Fuchs-Baumgartinger"
    },
    {
      "affs": [
        0
      ],
      "name": "Andrea Klang",
      "orcid": "0000-0001-5352-5757"
    },
    {
      "affs": [
        8
      ],
      "name": "Robert Klopfleisch",
      "orcid": "0000-0002-6308-0568"
    },
    {
      "affs": [
        0
      ],
      "name": "Barbara Richter"
    },
    {
      "affs": [
        9
      ],
      "name": "Marc Aubreville",
      "orcid": "0000-0002-5294-5247"
    },
    {
      "affs": [
        4
      ],
      "name": "Stephan M. Winkler"
    },
    {
      "affs": [
        10
      ],
      "name": "Matti Kiupel",
      "orcid": "0000-0003-4429-9529"
    },
    {
      "affs": [
        8
      ],
      "name": "Alexander Bartel",
      "orcid": "0000-0002-1280-6138"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/bxzb774gdvq4a.bib",
  "cited_by": 0,
  "doi": "10.1177/03009858261465447",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13714219/",
  "id": "bxzb774gdvq4a",
  "issns": [
    "0300-9858",
    "1544-2217"
  ],
  "landing_url": "https://doi.org/10.1177/03009858261465447",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Artificial Intelligence and Digital Pathology"
    ],
    [
      "heading",
      "Brief Communication"
    ],
    [
      "openalex",
      "2740|Veterinary Oncology Research"
    ]
  ],
  "openalex": "W7168181663",
  "pdf_url": null,
  "pmcid": "PMC13714219",
  "pmid": "42438334",
  "published": "2026-07-12",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/bxzb774gdvq4a.ris",
  "sources": [
    {
      "first_seen": "2026-11-01T12:21:45Z",
      "native_id": "PMC13714219",
      "source": "pmc",
      "url": "https://doi.org/10.1177/03009858261465447"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2740|Veterinary Oncology Research",
      "subfield": "general"
    }
  ],
  "title": "Intratumoral distribution of anisokaryosis in canine cutaneous mast cell tumors",
  "title_text": "Intratumoral distribution of anisokaryosis in canine cutaneous mast cell tumors",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T12:21:45Z",
  "url": "https://research.draconitic.com/paper/bxzb774gdvq4a",
  "venue": "Veterinary Pathology",
  "versions": []
}