{
  "abstract": "Background\n\nReactive oxygen species (ROS) regulate stem-cell function, but sustained exposure causes oxidative damage, mitochondrial dysfunction and exhaustion. Multilineage-differentiating Stress-Enduring (MUSE) cells, a rare SSEA-3⁺ MSC subset, tolerate ROS-rich environments, although the mechanisms remain unclear.Methods\n\nWe compared MUSE, Non-MUSE (SSEA-3⁻) and MSCs after H₂O₂ exposure, assessing ROS clearance, mtDNA integrity, 8-oxo-dG, antioxidant and BER responses, mitochondrial morphology/function, mitophagy and biogenesis, with focus on the PGC-1α/NRF2 axis. Three independent biological replicates were analyzed by imaging, qPCR, flow cytometry and biochemical assays.Results\n\nMUSE cells showed greater resistance to oxidative stress than MSCs and Non-MUSE cells. After H₂O₂, they displayed lower ROS accumulation (~15-25%), preserved mtDNA integrity, reduced 8-oxo-dG, rapid induction of antioxidant and BER-related genes, and maintenance of mitochondrial morphology, membrane potential and respiratory activity. MUSE cells also showed enhanced mitophagy, with >2-fold increased LAMP1-MitoTracker colocalization and induction of PINK1/phospho-Ub-S65, together with increased biogenesis, including ~45% higher COX-I/SDHA ratio, TFAM induction and PGC-1α activation at 48 h.Conclusion\n\nMUSE cells coordinate antioxidant defense, DNA repair, mitophagy and biogenesis through a remove-and-replace program that preserves mitochondrial function during oxidative stress.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Life Sciences, Health and Health Professions, Link Campus University, Rome, Italy.",
    "Department of Experimental Medicine, Luigi Vanvitelli Campania University, Naples, Italy.",
    "Department of Biotechnology, University of Samarra, Samarra, Iraq.",
    "Department of Medical Laboratory Sciences, Bahir Dar University, Bahir Dar, Ethiopia.",
    "Saint Camillus International University of Health and Medical Sciences, UNICAMILLUS, Rome, Italy.",
    "Genome and Stem Cell Center (GENKÖK), Erciyes University, Kayseri, Turkey."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Domenico Aprile",
      "orcid": "0000-0003-1166-3259"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Sura Hilal Ahmed Al-Sammarraie",
      "orcid": "0009-0002-2293-6080"
    },
    {
      "affs": [
        1
      ],
      "name": "Alessia Ambrosino",
      "orcid": "0009-0004-4371-7754"
    },
    {
      "affs": [
        1
      ],
      "name": "Mohd Shahzaib"
    },
    {
      "affs": [
        3
      ],
      "name": "Yesuf Siraj",
      "orcid": "0000-0001-8016-6817"
    },
    {
      "affs": [
        1
      ],
      "name": "Afshin Samiminemati"
    },
    {
      "affs": [
        1
      ],
      "name": "Nicola Alessio",
      "orcid": "0000-0002-5019-2130"
    },
    {
      "affs": [
        4
      ],
      "name": "Gianfranco Peluso",
      "orcid": "0000-0003-4352-9484"
    },
    {
      "affs": [
        1
      ],
      "name": "Giovanni Di Bernardo",
      "orcid": "0000-0002-4985-4029"
    },
    {
      "affs": [
        1,
        5
      ],
      "name": "Umberto Galderisi",
      "orcid": "0000-0003-0909-7403"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/byeb0ra8cdy51.bib",
  "cited_by": 0,
  "doi": "10.1080/13510002.2026.2698189",
  "fulltext_url": "https://europepmc.org/articles/PMC13656651",
  "id": "byeb0ra8cdy51",
  "issns": [
    "1351-0002",
    "1743-2928"
  ],
  "landing_url": "https://europepmc.org/article/MED/42852617",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Mitochondrial Function and Pathology"
    ]
  ],
  "openalex": "W7221139763",
  "pdf_url": "https://europepmc.org/articles/PMC13656651?pdf=render",
  "pmcid": "PMC13656651",
  "pmid": "42852617",
  "published": "2026-10-09",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/byeb0ra8cdy51.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:02Z",
      "native_id": "MED:42852617",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42852617"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Mitochondrial Function and Pathology",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Coordinated mitochondrial quality control underlies oxidative stress resilience in MUSE stem cells.",
  "title_text": "Coordinated mitochondrial quality control underlies oxidative stress resilience in MUSE stem cells.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:02Z",
  "url": "https://research.draconitic.com/paper/byeb0ra8cdy51",
  "venue": "Redox report : communications in free radical research",
  "versions": []
}