{
  "abstract": "Artemisinin-based Combination Therapies (ACTs) remain the first-line treatment for uncomplicated Plasmodium falciparum malaria in Senegal. The recent emergence of World Health Organization (WHO)-validated Pfkelch13 (K13) mutations associated with artemisinin partial resistance in Africa highlights the need for continuous molecular surveillance. We conducted a nationwidegenomic surveillance study of 3,800 P. falciparum isolates collected from nine malaria-endemic regions of Senegal during 2023-2024. Mutations in the K13 gene were detected using targeted amplicon deep sequencing, and mutations were independently confirmed by allele-specific quantitative PCR (qPCR). Among all isolates analyzed, a single sample collected in Kafountine, Ziguinchor Region, harbored the WHO-validated K13 C469Y mutation at a allele frequency of approximately 15%, indicating a minority mutant parasite population. Allele-specific qPCR confirmed the presence of the mutant allele, and epidemiologic investigation indicated the patient had no recent travel history outside the study area, suggesting local acquisition. No additional WHO-validated K13 resistance mutations were detected, and the patient recovered completely following treatment with artemether-lumefantrine. To our knowledge, this finding represents the first detection of the WHO-validated K13 C469Y mutation in Senegal. Although identified in a single mixed infection and not associated with clinical evidence of ACT failure, the detection of a validated artemisinin resistance mutation prompts deeper investigation and underscores the importance of integrating molecular surveillance into routine malaria control programs to enable early detection of emerging resistance to support evidence-based public health decision-making.",
  "abstract_shown": true,
  "affiliations": [
    "International Research & Training Center in Applied Genomics and Health Surveillance"
  ],
  "article_type": "PUBLISHAHEADOFPRINT",
  "arxiv": null,
  "author_total": 28,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "A. Gaye"
    },
    {
      "name": "B. Ngom"
    },
    {
      "name": "D. Sow"
    },
    {
      "name": "A. Sene"
    },
    {
      "name": "M. Toure"
    },
    {
      "name": "A. Tine"
    },
    {
      "name": "Y. D. Ndiaye"
    },
    {
      "name": "B. Dieye"
    },
    {
      "name": "K. Diongue"
    },
    {
      "name": "Y. Diedhiou"
    },
    {
      "name": "M. F. Ndiaye"
    },
    {
      "name": "M. Ndiaye"
    },
    {
      "name": "A. M. Mbaye"
    },
    {
      "name": "A. Thiongane"
    },
    {
      "name": "N. K. Standeur"
    },
    {
      "name": "L. Ndiaye"
    },
    {
      "name": "D. Zoumarou"
    },
    {
      "name": "J. F. Gomis"
    },
    {
      "name": "M. C. Seck"
    },
    {
      "name": "I. M. Ndiaye"
    },
    {
      "name": "I. Diallo"
    },
    {
      "name": "E. Doucoure"
    },
    {
      "name": "N. C. Diop"
    },
    {
      "name": "A. K. Dia"
    },
    {
      "name": "A. S. Badiane"
    },
    {
      "name": "M. A. Diallo"
    },
    {
      "name": "A. B. Deme"
    },
    {
      "name": "D. Ndiaye"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/bznnfcv0m82kr.bib",
  "cited_by": 1,
  "doi": "10.64898/2026.09.11.26362519",
  "fulltext_url": "https://www.medrxiv.org/content/10.64898/2026.09.11.26362519v1.full",
  "id": "bznnfcv0m82kr",
  "issns": [],
  "landing_url": "https://www.medrxiv.org/content/10.64898/2026.09.11.26362519v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "medrxiv",
      "infectious diseases"
    ],
    [
      "openalex",
      "2739|Malaria Research and Control"
    ]
  ],
  "openalex": "W7212381390",
  "pdf_url": "https://www.medrxiv.org/content/10.64898/2026.09.11.26362519v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-11",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/bznnfcv0m82kr.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:25Z",
      "native_id": "10.64898/2026.09.11.26362519",
      "source": "medrxiv",
      "url": "https://www.medrxiv.org/content/10.64898/2026.09.11.26362519v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "medrxiv:infectious diseases",
      "subfield": "infectious-disease"
    }
  ],
  "title": "First Detection of WHO-validated artemisinin resistance Plasmodium falciparum K13 C469Y mutation in patient from Senegal.",
  "title_text": "First Detection of WHO-validated artemisinin resistance Plasmodium falciparum K13 C469Y mutation in patient from Senegal.",
  "type": "preprint",
  "updated_at": "2026-10-30T12:18:25Z",
  "url": "https://research.draconitic.com/paper/bznnfcv0m82kr",
  "venue": "medRxiv",
  "versions": []
}