{
  "abstract": "Leaf-level optical measurements are essential for monitoring Mediterranean riparian forests. This study used visible spectroscopy (400-750 nm) to determine and quantify leaf pigments in five species: Salix salviifolia, Fraxinus angustifolia, Alnus glutinosa, Populus nigra, and Prunus avium. We followed Hansmann's method for sample processing and quantified chlorophyll using Arnon's equation. We collected samples from sun-exposed leaves in spring under standardized conditions. Results indicated that Alnus glutinosa and Populus nigra exhibited the highest chlorophyll a concentrations [1.35 and 1.29 mg g⁻¹ (FM)], followed by Fraxinus angustifolia [1.17 mg g⁻¹(FM)], Salix salviifolia [0.61 mg g⁻¹(FM)], and Prunus avium [0.25 mg g⁻¹(FM)]. The chlorophyll a/b ratio exceeded 2.0 in most species, peaking in Salix salviifolia (2.90). These findings reveal significant functional differences in pigment composition, confirming the utility of this method for monitoring the physiological status of the vegetation in the Manzanares River, Spain.",
  "abstract_shown": true,
  "affiliations": [
    "Hydrobiology Group, Department of Systems and Natural Resources, Higher Technical School of Forestry, Forest and Natural Environment, Polytechnic University of Madrid, University City, 28040 Madrid, Spain.",
    "National Polytechnic Institute, Higher School of Economics, Plan de Agua Prieta 66, Plutarco Elías Calles, Miguel Hidalgo, 11350 Mexico City, Mexico."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "M Martínez Mena",
      "orcid": "0000-0003-0328-9578"
    },
    {
      "affs": [
        1
      ],
      "name": "M D L Soto Rosales",
      "orcid": "0000-0002-2446-7161"
    },
    {
      "affs": [
        0
      ],
      "name": "D García de Jalón",
      "orcid": "0000-0003-0495-0700"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/bztec9pagzrqy.bib",
  "cited_by": 0,
  "doi": "10.32615/ps.2026.033",
  "fulltext_url": "https://europepmc.org/articles/PMC13725803",
  "id": "bztec9pagzrqy",
  "issns": [
    "0300-3604",
    "1573-9058"
  ],
  "landing_url": "https://europepmc.org/article/MED/42974300",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research Paper"
    ],
    [
      "openalex",
      "2303|Remote Sensing in Agriculture"
    ]
  ],
  "openalex": "W7221584528",
  "pdf_url": "https://europepmc.org/articles/PMC13725803?pdf=render",
  "pmcid": "PMC13725803",
  "pmid": "42974300",
  "published": "2026-10-13",
  "publisher": "Institute of Experimental Botany ASCR",
  "ris": "https://research.draconitic.com/paper/bztec9pagzrqy.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T15:20:49Z",
      "native_id": "MED:42974300",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42974300"
    },
    {
      "first_seen": "2026-11-05T12:22:16Z",
      "native_id": "PMC13725803",
      "source": "pmc",
      "url": "https://doi.org/10.32615/ps.2026.033"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2303|Remote Sensing in Agriculture",
      "subfield": "ecology"
    }
  ],
  "title": "Determination of chlorophyll and carotenoid content in Mediterranean riparian forest species using visible spectroscopy.",
  "title_text": "Determination of chlorophyll and carotenoid content in Mediterranean riparian forest species using visible spectroscopy.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-06T12:21:54Z",
  "url": "https://research.draconitic.com/paper/bztec9pagzrqy",
  "venue": "Photosynthetica",
  "versions": []
}